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Application of molecular fingerprinting for analysis of a PAH-contaminated soil microbiota growing in the presence of complex PAHs


Silva, IS and De Menezes, CR and De Faria, AF and Da Costa, JG and Pepper, S and Britz, ML and Durrant, LR, Application of molecular fingerprinting for analysis of a PAH-contaminated soil microbiota growing in the presence of complex PAHs, Acta Scientiarum. Biological Sciences , 32, (1) pp. 63-69. ISSN 1679-9283 (2010) [Refereed Article]


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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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DOI: doi:10.4025/actascibiolsci.v32i1.7575


Polycyclic aromatic hydrocarbons (PAHs) constitute a group of priority pollutants which are present at high concentrations in the soils of many industrial contaminated sites. Pollution by these compounds may stimulate growth of organisms able to live in these environments causing changes in the structure of the microbial community due to some cooperative process of metabolization of toxic compounds. A long-term PAH-contaminated soil was stored for several years and used to analyze the native microbiota regarding their ability to grow on pyrene, benzo[a]pyrene, as well as in mixtures of LMW- and HMW-PAHs. Molecular profiles of the microbial community was assessed by PCR-DGGE of 16S rRNA gene, and the number of bands observed in DGGE analyses was interpreted as dominant microbial members into the bacterial community. Results of PAH-contaminated soil microorganisms showed different profiles in the degradative dynamics when some nutrients were added. Predominant species may play a significative role while growing and surviving on PAHs, and some other metabolically active species have emerged to interact themselves in a cooperative catabolism of PAHs.

Item Details

Item Type:Refereed Article
Research Division:Environmental Sciences
Research Group:Environmental biotechnology
Research Field:Bioremediation
Objective Division:Environmental Management
Objective Group:Terrestrial systems and management
Objective Field:Evaluation, allocation, and impacts of land use
UTAS Author:Britz, ML (Professor Margaret Britz)
ID Code:70183
Year Published:2010
Deposited By:Agricultural Science
Deposited On:2011-06-07
Last Modified:2011-07-01
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