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Determination of Albuterol Enantiomers in Animal Tissue Matrices by LC-MS/MS: Application in Therapeutic Myoanabolic Studies

Citation

Yee, KC and Lowe, E and Jacobson, GA, Determination of Albuterol Enantiomers in Animal Tissue Matrices by LC-MS/MS: Application in Therapeutic Myoanabolic Studies, Chromatographia, 74, (3-4) pp. 355-358. ISSN 0009-5893 (2011) [Refereed Article]


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The final publication is available at http://www.springerlink.com

DOI: doi:10.1007/s10337-011-2061-z

Abstract

Albuterol (salbutamol) is a widely used medication in respiratory disease including asthma and chronic obstructive airways disease; however, like other beta2- agonists, it also exerts some extrapulmonary effects on muscle and fat. Surprisingly, there have been relatively few reports of albuterol tissue distribution, and the distribution of individual albuterol enantiomers into tissue has not been reported. The method presented here explores the use of an HPLC tandem mass spectrometry (LC–MS/MS) system (LTQ Orbitrap hybrid mass spectrometer) with deuterated standard and solid-phase extraction to determine low levels of albuterol enantiomers in tissue. The lower limit of quantification (LLOQ) was 0.156 ng g-1, with a precision RSD \15% for both enantiomers. The assay was linear over the calibration range of LLOQ-10.0 ng g-1 in muscle tissue (r2[0.98). The assay was successfully applied to pharmacokinetic studies in rats and mice. By utilizing a deuterated internal standard and LC–MS/MS detection, this assay can be used to measure albuterol enantiomers in muscle tissue. This assay has also identified that albuterol uptake appears to be stereoselective, and enantioselective assays are clearly warranted for myoanabolic studies involving administration of racemic-albuterol.

Item Details

Item Type:Refereed Article
Keywords:Salbutamol; Chiral; Animal tissue; Liquid chromatography-tandem mass spectrometry
Research Division:Medical and Health Sciences
Research Group:Pharmacology and Pharmaceutical Sciences
Research Field:Clinical Pharmacology and Therapeutics
Objective Division:Health
Objective Group:Other Health
Objective Field:Health not elsewhere classified
Author:Yee, KC (Dr Kwang Yee)
Author:Lowe, E (Mr Edwin Lowe)
Author:Jacobson, GA (Dr Glenn Jacobson)
ID Code:70114
Year Published:2011
Web of Science® Times Cited:6
Deposited By:Pharmacy
Deposited On:2011-06-03
Last Modified:2017-11-02
Downloads:0

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