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Unexpected property of ectoine synthase and its application for synthesis of the engineered compatible solute ADPC
Citation
Witt, EMHJ and Davies, NW and Galinski, EA, Unexpected property of ectoine synthase and its application for synthesis of the engineered compatible solute ADPC , Applied Microbiology and Biotechnology, 91, (1) pp. 113-122. ISSN 0175-7598 (2011) [Refereed Article]
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Copyright Statement
Copyright 2011 Springer-Verlag
DOI: doi:10.1007/s00253-011-3211-9
Abstract
A new cyclic amino acid was detected in a deletion
mutant of the moderately halophilic bacterium Halomonas
elongata deficient in ectoine synthesis. Using mass spectroscopy
(MS) and nuclear magnetic resonance (NMR)
techniques, the substance was identified as 5-amino-3,4-
dihydro-2H-pyrrole-2-carboxylate (ADPC). We were able to
demonstrate that ADPC is the product of a side reaction of
lone ectoine synthase (EC 4.2.1.108), which forms ADPC
by cyclic condensation of glutamine. This reaction was
shown to be reversible. Subsequently, a number of ectoine
derivatives, in particular 4,5-dihydro-2-methylimidazole-4-
carboxylate (DHMICA) and homoectoine, were also shown
to be cleaved by ectoine synthase, which is classified as a
hydro-lyase. This study thus reports for the first time that
ectoine synthase accepts more than one substrate and is a
reversible enzyme able to catalyze both the intramolecular
condensation into and the hydrolytic cleavage of cyclic amino
acid derivatives. As ADPC supports growth of bacteria under
salt stress conditions and stabilizes enzymes against freezethaw
denaturation, it displays typical properties of compatible
solutes. As ADPC has not yet been described as a natural
compound, it is presented here as the first man-made
compatible solute created through genetic engineering.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | Halomonas elongata . Ectoine synthase . ADPC . Genetic engineering . Cyclic amino acid derivatives . Compatible solute |
Research Division: | Biological Sciences |
Research Group: | Microbiology |
Research Field: | Bacteriology |
Objective Division: | Health |
Objective Group: | Public health (excl. specific population health) |
Objective Field: | Food safety |
UTAS Author: | Davies, NW (Associate Professor Noel Davies) |
ID Code: | 70083 |
Year Published: | 2011 |
Web of Science® Times Cited: | 18 |
Deposited By: | Central Science Laboratory |
Deposited On: | 2011-05-31 |
Last Modified: | 2017-11-06 |
Downloads: | 1 View Download Statistics |
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