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Olfactory ensheathing cells moderate nuclear factor kappaB translocation in astrocytes
Citation
Hale, DM and Ray, S and Leung, JY and Holloway, AF and Chung, RS and West, AK and Chuah, MI, Olfactory ensheathing cells moderate nuclear factor kappaB translocation in astrocytes , Molecular and Cellular Neuroscience, 46, (1) pp. 213-221. ISSN 1044-7431 (2011) [Refereed Article]
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DOI: doi:10.1016/j.mcn.2010.09.004
Abstract
Nuclear factor kappaB (NFêB) is a key transcriptional regulator of inflammatory genes. We investigated the
modulatory effects of olfactory ensheathing cells (OECs),microglia and meningeal fibroblasts on translocation
of NFêB to astrocyte nuclei. The percentage of activated astrocytes in co-cultures with OECs was significantly
less than for co-cultures withmicroglia (pb0.001) and fibroblasts (pb0.05). Phorbol myristate acetate (PMA)
and calcium ionophore stimulation of p65 NFêB translocation to nuclei provided an in vitro model of astrocyte
inflammatory activation. Soluble factors released by OECs significantly moderated the astrocytic NFêB
translocation induced by either PMA/calcium ionophore or microglia-derived factors (pb0.001). Insulin-like
growth factor-1 may contribute to these effects, since it is expressed by OECs and also significantly moderated
the astrocytic NFêB translocation (pb0.05), albeit insufficiently to fully account for the OEC-induced
moderation (pb0.01). Olfactory ensheathing cells significantly moderated the increased transcription of the
pro-inflammatory cytokine, granulocyte macrophage-colony stimulating factor in the activated astrocytes
(pb0.01). These results suggest that transplanted OECs could improve neural repair after CNS injury by
moderating astrocyte activation.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Olfactory ensheathing cells; Activation of astrocytes; Nuclear factor kappaB; Microglia; Inflammation |
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Neurosciences |
| Research Field: | Cellular nervous system |
| Objective Division: | Health |
| Objective Group: | Clinical health |
| Objective Field: | Clinical health not elsewhere classified |
| UTAS Author: | Hale, DM (Mr David Hale) |
| UTAS Author: | Ray, S (Mrs Shannon Huskins) |
| UTAS Author: | Leung, JY (Dr Jacqueline Leung) |
| UTAS Author: | Holloway, AF (Professor Adele Holloway) |
| UTAS Author: | Chung, RS (Associate Professor Roger Chung) |
| UTAS Author: | West, AK (Professor Adrian West) |
| UTAS Author: | Chuah, MI (Associate Professor Inn Chuah) |
| ID Code: | 69668 |
| Year Published: | 2011 |
| Web of Science® Times Cited: | 8 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2011-05-12 |
| Last Modified: | 2012-03-19 |
| Downloads: | 0 |
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