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Olfactory ensheathing cells moderate nuclear factor kappaB translocation in astrocytes


Hale, DM and Ray, S and Leung, JY and Holloway, AF and Chung, RS and West, AK and Chuah, MI, Olfactory ensheathing cells moderate nuclear factor kappaB translocation in astrocytes , Molecular and Cellular Neuroscience, 46, (1) pp. 213-221. ISSN 1044-7431 (2011) [Refereed Article]

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DOI: doi:10.1016/j.mcn.2010.09.004


Nuclear factor kappaB (NFB) is a key transcriptional regulator of inflammatory genes. We investigated the modulatory effects of olfactory ensheathing cells (OECs),microglia and meningeal fibroblasts on translocation of NFB to astrocyte nuclei. The percentage of activated astrocytes in co-cultures with OECs was significantly less than for co-cultures withmicroglia (pb0.001) and fibroblasts (pb0.05). Phorbol myristate acetate (PMA) and calcium ionophore stimulation of p65 NFB translocation to nuclei provided an in vitro model of astrocyte inflammatory activation. Soluble factors released by OECs significantly moderated the astrocytic NFB translocation induced by either PMA/calcium ionophore or microglia-derived factors (pb0.001). Insulin-like growth factor-1 may contribute to these effects, since it is expressed by OECs and also significantly moderated the astrocytic NFB translocation (pb0.05), albeit insufficiently to fully account for the OEC-induced moderation (pb0.01). Olfactory ensheathing cells significantly moderated the increased transcription of the pro-inflammatory cytokine, granulocyte macrophage-colony stimulating factor in the activated astrocytes (pb0.01). These results suggest that transplanted OECs could improve neural repair after CNS injury by moderating astrocyte activation.

Item Details

Item Type:Refereed Article
Keywords:Olfactory ensheathing cells; Activation of astrocytes; Nuclear factor kappaB; Microglia; Inflammation
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Cellular nervous system
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Hale, DM (Mr David Hale)
UTAS Author:Ray, S (Mrs Shannon Huskins)
UTAS Author:Leung, JY (Dr Jacqueline Leung)
UTAS Author:Holloway, AF (Professor Adele Holloway)
UTAS Author:Chung, RS (Associate Professor Roger Chung)
UTAS Author:West, AK (Professor Adrian West)
UTAS Author:Chuah, MI (Associate Professor Inn Chuah)
ID Code:69668
Year Published:2011
Web of Science® Times Cited:8
Deposited By:Menzies Institute for Medical Research
Deposited On:2011-05-12
Last Modified:2012-03-19

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