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Altered dopamine receptor and dopamine transporter binding and tyrosine hydroxylase mRNA expression following perinatal NMDA receptor blockade
journal contribution
posted on 2023-05-17, 06:02 authored by du Bois, TM, Hsu, CW, Li, Y, Tan, YY, Deng, C, Huang, XFThis study examined how perinatal phencyclidine (PCP) treatment would affect dopamine D2 receptor and dopamine transporter (DAT) binding at different stages after treatment cessation. Female rat pups received injections of PCP (10 mg/kg, s.c.) or saline on postnatal day (PN)7, 9 and 11. D2 receptor and transporter binding was examined at four time-points (PN12, 18, 32 and 96) following injections. PCP treatment altered D2 receptor binding throughout development, with a final end-point of 22-33% decreased binding at adulthood in the nucleus accumbens and caudate putamen (P\0.01), accompanied by a small but significant increase in DAT binding in the caudate putamen. Tyrosine hydroxylase mRNA expression was also significantly increased by 25% (P\0.05) in the ventral tegmental area of adult rats, suggesting that this model may produce a long-term increase in dopamine output. This study demonstrates that early insult to the brain from NMDA receptor hypofunction alters the dopaminergic system at different stages of development.
History
Publication title
Neurochemical ResearchVolume
33Issue
7Pagination
1224-1231ISSN
0364-3190Department/School
School of Pharmacy and PharmacologyPublisher
Kluwer Academic/Plenum PublPlace of publication
233 Spring St, New York, USA, Ny, 10013Rights statement
The final publication is available at http://www.springerlink.comRepository Status
- Restricted