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Effects of antiphospholipid antibodies on in vitro platelet aggregation

Citation

Palatinus, AA and Ahuja, KDK and Adams, MJ, Effects of antiphospholipid antibodies on in vitro platelet aggregation, Clinical and Applied Thrombosis/Hemostasis, 18, (1) pp. 59-65. ISSN 1076-0296 (2012) [Refereed Article]

Copyright Statement

Copyright 2012 SAGE

DOI: doi:10.1177/1076029611412360

Abstract

Antiphospholipid antibodies contribute to the development of thrombosis, although precise mechanisms remain to be elucidated. We determined the effects of affinity-purified anti-beta2-glycoprotein 1 (anti-β2GP1) and anti-prothrombin (anti-PT) antibodies on in vitro platelet aggregation. Adenosine diphosphate (ADP) and collagen-induced platelet aggregation were performed using platelet-rich plasma ([PRP] 250 × 109/L). Antiphospholipid antibodies (1.25-10 μg/mL) were preincubated with PRP for 10 minutes at 37°C prior to the addition of agonist. Anti-β2GP1 antibodies significantly reduced platelet aggregation (percentage area under the curve; %AUC) in a concentration-dependent manner using both 5 μmol/L (P < .001) and 2.5 μmol/L (P = .038) ADP but did not significantly affect the rate of aggregation. Anti-PT antibodies significantly enhanced 5 µg/mL collagen-induced platelet aggregation (%AUC; P = .034) but did not affect ADP-induced platelet aggregation. These results suggest (1) interactions and effects of antiphospholipid antibodies on platelets are agonist and concentration dependent and (2) anti-β2GP1 antibodies may inhibit dense granule release and/or inhibition of the arachidonic acid pathway.

Item Details

Item Type:Refereed Article
Keywords:antiphospholipid antibody, platelets, thrombosis, aggregation, anti-b2GP1
Research Division:Medical and Health Sciences
Research Group:Cardiorespiratory Medicine and Haematology
Research Field:Haematology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Blood Disorders
UTAS Author:Palatinus, AA (Miss Annita Palatinus)
UTAS Author:Ahuja, KDK (Dr Kiran Ahuja)
UTAS Author:Adams, MJ (Dr Murray Adams)
ID Code:68974
Year Published:2012
Web of Science® Times Cited:6
Deposited By:Health Sciences A
Deposited On:2011-04-11
Last Modified:2017-11-03
Downloads:0

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