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Effects of antiphospholipid antibodies on in vitro platelet aggregation


Palatinus, AA and Ahuja, KDK and Adams, MJ, Effects of antiphospholipid antibodies on in vitro platelet aggregation, Clinical and Applied Thrombosis/Hemostasis, 18, (1) pp. 59-65. ISSN 1076-0296 (2012) [Refereed Article]

Copyright Statement

Copyright 2012 SAGE

DOI: doi:10.1177/1076029611412360


Antiphospholipid antibodies contribute to the development of thrombosis, although precise mechanisms remain to be elucidated. We determined the effects of affinity-purified anti-beta2-glycoprotein 1 (anti-β2GP1) and anti-prothrombin (anti-PT) antibodies on in vitro platelet aggregation. Adenosine diphosphate (ADP) and collagen-induced platelet aggregation were performed using platelet-rich plasma ([PRP] 250 × 109/L). Antiphospholipid antibodies (1.25-10 μg/mL) were preincubated with PRP for 10 minutes at 37°C prior to the addition of agonist. Anti-β2GP1 antibodies significantly reduced platelet aggregation (percentage area under the curve; %AUC) in a concentration-dependent manner using both 5 μmol/L (P < .001) and 2.5 μmol/L (P = .038) ADP but did not significantly affect the rate of aggregation. Anti-PT antibodies significantly enhanced 5 µg/mL collagen-induced platelet aggregation (%AUC; P = .034) but did not affect ADP-induced platelet aggregation. These results suggest (1) interactions and effects of antiphospholipid antibodies on platelets are agonist and concentration dependent and (2) anti-β2GP1 antibodies may inhibit dense granule release and/or inhibition of the arachidonic acid pathway.

Item Details

Item Type:Refereed Article
Keywords:antiphospholipid antibody, platelets, thrombosis, aggregation, anti-b2GP1
Research Division:Biomedical and Clinical Sciences
Research Group:Cardiovascular medicine and haematology
Research Field:Haematology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Diagnosis of human diseases and conditions
UTAS Author:Palatinus, AA (Miss Annita Palatinus)
UTAS Author:Ahuja, KDK (Dr Kiran Ahuja)
UTAS Author:Adams, MJ (Dr Murray Adams)
ID Code:68974
Year Published:2012
Web of Science® Times Cited:9
Deposited By:Health Sciences A
Deposited On:2011-04-11
Last Modified:2017-11-03

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