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Utility of gel-free, label-free shotgun proteomics approaches to investigate microorganisms


Porteus, B and Kocharunchitt, C and Nilsson, RE and Ross, T and Bowman, JP, Utility of gel-free, label-free shotgun proteomics approaches to investigate microorganisms, Applied Microbiology and Biotechnology, 90, (2) pp. 407-416. ISSN 0175-7598 (2011) [Refereed Article]

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DOI: doi:10.1007/s00253-011-3172


This review will examine the current situation with label-free, quantitative, shotgun-oriented proteomics technology and discuss the advantages and limitations associated with its capability in capturing and quantifying large portions of proteomes of microorganisms. Such an approach allows (1) comparisons between physiological or genetic states of organisms at the protein level, (2) ‘painting’ of proteomic data onto genome data-based metabolic maps, (3) enhancement of the utility of genomic data and finally (4) surveying of non-genome sequenced microorganisms by taking advantage of available inferred protein data in order to gain new insights into straindependent metabolic or physiological capacities. The technology essentially is a powerful addition to systems biology with a capacity to be used to ask hypothesis-driven ‘top-down’ questions or for more empirical ‘bottom-up’ exploration.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Proteomics and intermolecular interactions (excl. medical proteomics)
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Porteus, B (Miss Bianca Porteus)
UTAS Author:Kocharunchitt, C (Mr Chawalit Kocharunchitt)
UTAS Author:Nilsson, RE (Mr Rolf Nilsson)
UTAS Author:Ross, T (Professor Tom Ross)
UTAS Author:Bowman, JP (Associate Professor John Bowman)
ID Code:68955
Year Published:2011
Web of Science® Times Cited:21
Deposited By:Agricultural Science
Deposited On:2011-04-06
Last Modified:2015-02-07

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