Dai, F and Qiu, L and Ye, L and Wu, D and Zhou, M and Zhang, G, Identification of a phytase gene in barley (Hordeum vulgare L.), PLoS One, 6, (4) Article e18829. ISSN 1932-6203 (2011) [Refereed Article]
Copyright © 2011 Dai et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Official URL: http://www.plosone.org/article/info%3Adoi%2F10.137...
Background: Endogenous phytase plays a crucial role in phytate degradation and is thus closely related to nutrient efficiency in barley products. The understanding of genetic information of phytase in barley can provide a useful tool for breeding new barley varieties with high phytase activity.
Methodology/Principal Findings: Quantitative trait loci (QTL) analysis for phytase activity was conducted using a doubled haploid population. Phytase protein was purified and identified by the LC-ESI MS/MS Shotgun method. Purple acid phosphatase (PAP) gene was sequenced and the position was compared with the QTL controlling phytase activity. A major QTL for phytase activity was mapped to chromosome 5 H in barley. The gene controlling phytase activity in the region was named as mqPhy. The gene HvPAP a was mapped to the same position as mqPhy, supporting the colinearity between HvPAP a and mqPhy.
Conclusions/Significance: It is the first report on QTLs for phytase activity and the results showed that HvPAP a, which shares a same position with the QTL, is a major phytase gene in barley grains.
|Item Type:||Refereed Article|
|Research Division:||Agricultural and Veterinary Sciences|
|Research Group:||Crop and Pasture Production|
|Research Field:||Crop and Pasture Improvement (Selection and Breeding)|
|Objective Division:||Plant Production and Plant Primary Products|
|Objective Group:||Winter Grains and Oilseeds|
|UTAS Author:||Zhou, M (Professor Meixue Zhou)|
|Web of Science® Times Cited:||11|
|Deposited By:||Agricultural Science|
|Downloads:||429 View Download Statistics|
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