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Identification of a phytase gene in barley (Hordeum vulgare L.)


Dai, F and Qiu, L and Ye, L and Wu, D and Zhou, M and Zhang, G, Identification of a phytase gene in barley (Hordeum vulgare L.), PLoS One, 6, (4) Article e18829. ISSN 1932-6203 (2011) [Refereed Article]


Copyright Statement

Copyright 2011 Dai et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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DOI: doi:10.1371/journal.pone.0018829


Background: Endogenous phytase plays a crucial role in phytate degradation and is thus closely related to nutrient efficiency in barley products. The understanding of genetic information of phytase in barley can provide a useful tool for breeding new barley varieties with high phytase activity.

Methodology/Principal Findings: Quantitative trait loci (QTL) analysis for phytase activity was conducted using a doubled haploid population. Phytase protein was purified and identified by the LC-ESI MS/MS Shotgun method. Purple acid phosphatase (PAP) gene was sequenced and the position was compared with the QTL controlling phytase activity. A major QTL for phytase activity was mapped to chromosome 5 H in barley. The gene controlling phytase activity in the region was named as mqPhy. The gene HvPAP a was mapped to the same position as mqPhy, supporting the colinearity between HvPAP a and mqPhy.

Conclusions/Significance: It is the first report on QTLs for phytase activity and the results showed that HvPAP a, which shares a same position with the QTL, is a major phytase gene in barley grains.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Improvement (Selection and Breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Barley
Author:Zhou, M (Professor Meixue Zhou)
ID Code:68691
Year Published:2011
Web of Science® Times Cited:8
Deposited By:Agricultural Science
Deposited On:2011-03-17
Last Modified:2017-11-06
Downloads:334 View Download Statistics

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