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Identification of a phytase gene in barley (Hordeum vulgare L.)

Citation

Dai, F and Qiu, L and Ye, L and Wu, D and Zhou, M and Zhang, G, Identification of a phytase gene in barley (Hordeum vulgare L.), PLoS One, 6, (4) Article e18829. ISSN 1932-6203 (2011) [Refereed Article]


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Copyright Statement

Copyright 2011 Dai et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Official URL: http://www.plosone.org/article/info%3Adoi%2F10.137...

DOI: doi:10.1371/journal.pone.0018829

Abstract

Background: Endogenous phytase plays a crucial role in phytate degradation and is thus closely related to nutrient efficiency in barley products. The understanding of genetic information of phytase in barley can provide a useful tool for breeding new barley varieties with high phytase activity.

Methodology/Principal Findings: Quantitative trait loci (QTL) analysis for phytase activity was conducted using a doubled haploid population. Phytase protein was purified and identified by the LC-ESI MS/MS Shotgun method. Purple acid phosphatase (PAP) gene was sequenced and the position was compared with the QTL controlling phytase activity. A major QTL for phytase activity was mapped to chromosome 5 H in barley. The gene controlling phytase activity in the region was named as mqPhy. The gene HvPAP a was mapped to the same position as mqPhy, supporting the colinearity between HvPAP a and mqPhy.

Conclusions/Significance: It is the first report on QTLs for phytase activity and the results showed that HvPAP a, which shares a same position with the QTL, is a major phytase gene in barley grains.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Improvement (Selection and Breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Barley
Author:Zhou, M (Associate Professor Meixue Zhou)
ID Code:68691
Year Published:2011
Web of Science® Times Cited:8
Deposited By:Agricultural Science
Deposited On:2011-03-17
Last Modified:2017-11-06
Downloads:330 View Download Statistics

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