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Purine and pyrimidine nucleotide metabolism of vascular smooth muscle cells in culture


James, SG and Appleby, GJ and Miller, KAL and Steen, JT and Colquhoun, EQ and Clark, MG, Purine and pyrimidine nucleotide metabolism of vascular smooth muscle cells in culture, General Pharmacology, 27, (5) pp. 837-844. ISSN 0306-3623 (1996) [Refereed Article]

DOI: doi:10.1016/0306-3623(95)02087-X


1. Cultures of vascular smooth muscle cells accumulate extracellular breakdown products of purine and pyrimidine nucleotides that, over 9 hr, represent 60 ± 7 and 78 ± 17%, respectively, of the intracellular nucleotide content. 2. The accumulation is stimulated during contracture with 20 mM KCl or 70 μM carbachol, consistent with the notion that both pyrimidine and purine nucleotides are involved in the energetics of smooth muscle contracture. 3. Because the intracellular levels of pyrimidine and purine nucleotides remain constant, it appears likely that rates of synthesis match the rates of release. 4. Ectonucleotidases are present that can degrade ATP, UTP, and CTP. High-energy nucleotides may be the primary products released.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Medical physiology
Research Field:Cell physiology
Objective Division:Health
Objective Group:Other health
Objective Field:Other health not elsewhere classified
UTAS Author:Appleby, GJ (Mr Geoffrey Appleby)
UTAS Author:Miller, KAL (Ms Miller)
UTAS Author:Steen, JT (Dr John Steen)
UTAS Author:Colquhoun, EQ (Dr Eric Colquhoun)
UTAS Author:Clark, MG (Professor Michael Clark)
ID Code:6859
Year Published:1996
Web of Science® Times Cited:6
Deposited By:Biochemistry
Deposited On:1996-08-01
Last Modified:2011-08-16

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