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Considerations for the use of salivary IgA for monitoring mucosal immune function


Dwyer, DB and Booth, CK and Pacque, PF and Ball, MJ, Considerations for the use of salivary IgA for monitoring mucosal immune function, Aviation, Space, and Environmental Medicine, 81, (6) pp. 581-584. ISSN 0095-6562 (2010) [Refereed Article]

DOI: doi:10.3357/ASEM.2705.2010


Introduction: We aimed to make recommendations concerning the use of total IgA in saliva (s-IgA) as an aid for monitoring athletic and military training. Methods: Unstimulated whole saliva was collected from 16 subjects (11 women and 5 men ages 18-57) during nonconsecutive days of fasting and non-fasting. Seven samples were collected from each subject at 0700, 0900, 1200, 1400, 1600, 1800, and 2030 on each day and a further three samples were collected 30 min after three meals on the non-fasting day (at 0730, 1230, and 1830). Strenuous activity was avoided and subjects did not drink caffeine or alcohol-containing beverages. Albumin and s-IgA were measured by commercial nephelometric immunoassays with intra-analytical coefficient of variance (CVA) of 1.8% and 2.9%, respectively. Individual and group variations were determined. Diurnal variation was determined by use of repeated-measures analysis of variance. Results: CV-individual (CV1) was 48% for s-IgA concentration and 43% for s-IgA secretion and s-IgA:albumin. CV-group (CV C) for these same measures was 68%, 75%, and 68%, respectively. When measurements were adjusted for saliva flow rates there was no evidence that s-IgA is subject to diurnal variation. There was strong evidence for a postprandial decrease in s-IgA for all measures. Conclusion: The high degree of individuality in s-IgA precludes the use of population reference ranges for identifying individual abnormal results. For the purpose of monitoring individuals we recommend using the individual's calculated biological variance (determined from previous serial measurements over a period of days to weeks). Individual abnormal results can then be identified. © by the Aerospace Medical Association.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Immunology
Research Field:Humoural immunology and immunochemistry
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Dwyer, DB (Dr Dan Dwyer)
UTAS Author:Booth, CK (Dr Christine Booth)
UTAS Author:Pacque, PF (Dr Paul Pacque)
UTAS Author:Ball, MJ (Professor Madeleine Ball)
ID Code:68379
Year Published:2010
Web of Science® Times Cited:4
Deposited By:Health Sciences A
Deposited On:2011-03-11
Last Modified:2011-05-16

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