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Co-expression of SOX9 and SOX10 during melanocytic differentiation in vitro. Experimental Cell Research

Citation

Cook, AL and Smith, AG and Smit, DJ and Leonard, JH and Sturm, RA, Co-expression of SOX9 and SOX10 during melanocytic differentiation in vitro. Experimental Cell Research, Experimental Cell Research, 308, (1) pp. 222-235. ISSN 0014-4827 (2005) [Refereed Article]

DOI: doi:10.1016/j.yexcr.2005.04.019

Abstract

Investigations into pigment cell biology have relied on the ability to culture both murine and human melanocytes, numerous melanoma cell lines and more recently, murine and human melanoblasts. Melanoblast culture requires medium supplemented with a range of growth factors including Stem Cell Factor, Endothelin-3 and Fibroblast Growth Factor-2, withdrawal of which causes the cells to differentiate into melanocytes. Using the human melanoblast culture system, we have now examined the expression and/or DNA binding activity of several transcription factors implicated in melanocytic development and differentiation. Of these, the POU domain factor BRN2 and the SOX family member SOX10 are both highly expressed in unpigmented melanocyte precursors but are down-regulated upon differentiation. In contrast, the expression levels of the previously described MITF and PAX3 transcription factors remain relatively constant during the melanoblast–melanocyte transition. Moreover, BRN2 ablated melanoma cells lack expression of SOX10 and MITF but retain PAX3. A novel finding implicates a second SOX protein, SOX9, as a potential melanogenic transcriptional regulator, as its expression level is increased following the down-regulation of BRN2 and SOX10 in differentiated melanoblasts. Our results suggest that a complex network of transcription factor interactions requiring proper temporal coordination is necessary for acquisition and maintenance of the melanocytic phenotype.

Item Details

Item Type:Refereed Article
Keywords:POU; SOX; PAX; MITF; EDNRB; Differentiation; Melanoma
Research Division:Medical and Health Sciences
Research Group:Oncology and Carcinogenesis
Research Field:Cancer Genetics
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Cancer and Related Disorders
Author:Cook, AL (Dr Tony Cook)
ID Code:68245
Year Published:2005
Web of Science® Times Cited:45
Deposited By:Health Sciences A
Deposited On:2011-03-10
Last Modified:2011-03-10
Downloads:0

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