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Gene expression profiling reveals distinct expression patterns for Classic versus Variant Merkel cell phenotypes and new classifier genes to distinguish Merkel cell from small cell lung carcinoma

Citation

Ven Gele, M and Boyle, GM and Cook, AL and Vandesompele, J and Boonefaes, T and Rottiers, P and Van Roy, N and De Paepe, A and Parsons, PG and Leonard, JH and Speleman, F, Gene expression profiling reveals distinct expression patterns for Classic versus Variant Merkel cell phenotypes and new classifier genes to distinguish Merkel cell from small cell lung carcinoma, Oncogene, 23, (15) pp. 2732-2742. ISSN 0950-9232 (2004) [Refereed Article]

DOI: doi:10.1038/sj.onc.1207421

Abstract

Merkel cell carcinoma (MCC) is a rare aggressive skin tumor which shares histopathological and genetic features with small-cell lung carcinoma (SCLC), both are of neuroendocrine origin. Comparable to SCLC, MCC cell lines are classified into two different biochemical subgroups designated as 'Classic' and 'Variant'. With the aim to identify typical gene-expression signatures associated with these phenotypically different MCC cell lines subgroups and to search for differentially expressed genes between MCC and SCLC, we used cDNA arrays to profile 10 MCC cell lines and four SCLC cell lines. Using significance analysis of microarrays, we defined a set of 76 differentially expressed genes that allowed unequivocal identification of Classic and Variant MCC subgroups. We assume that the differential expression levels of some of these genes reflect, analogous to SCLC, the different biological and clinical properties of Classic and Variant MCC phenotypes. Therefore, they may serve as useful prognostic markers and potential targets for the development of new therapeutic interventions specific for each subgroup. Moreover, our analysis identified 17 powerful classifier genes capable of discriminating MCC from SCLC. Real-time quantitative RT–PCR analysis of these genes on 26 additional MCC and SCLC samples confirmed their diagnostic classification potential, opening opportunities for new investigations into these aggressive cancers.

Item Details

Item Type:Refereed Article
Keywords:Merkel cell carcinoma, small-cell lung carcinoma, differential expression profiling, differential diagnosis, Classic/Variant
Research Division:Medical and Health Sciences
Research Group:Oncology and Carcinogenesis
Research Field:Cancer Genetics
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Cancer and Related Disorders
Author:Cook, AL (Dr Tony Cook)
ID Code:68235
Year Published:2004
Web of Science® Times Cited:35
Deposited By:Health Sciences A
Deposited On:2011-03-10
Last Modified:2011-08-01
Downloads:0

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