eCite Digital Repository

Screening of human primary melanocytes of defined melanocortin-1 receptor genotype: pigmentation marker, ultrastructural and UV-survival studies

Citation

Leonard, JH and Marks, LH and Chen, W and Cook, AL and Boyle, GM and Smit, DJ and Brown, DL and Stow, JL and Parsons, PG and Sturm, JH, Screening of human primary melanocytes of defined melanocortin-1 receptor genotype: pigmentation marker, ultrastructural and UV-survival studies, Pigment Cell & Melanoma Research, 16, (3) pp. 198-207. ISSN 1755-148X (2003) [Refereed Article]

DOI: doi:10.1034/j.1600-0749.2003.00033.x

Abstract

Recent population studies have demonstrated an association with the red-hair and fair-skin phenotype with variant alleles of the melanocortin-1 receptor (MC1R) which result in amino acid substitutions within the coding region leading to an altered receptor activity. In particular, Arg151Cys, Arg160Trp and Asp294His were the most commonly associated variants seen in the south-east Queensland population with at least one of these alleles found in 93% of those with red hair. In order to study the individual effects of these variants on melanocyte biology and melanocytic pigmentation, we established a series of human melanocyte strains genotyped for the MC1R receptor which included wild-type consensus, variant heterozygotes, compound heterozygotes and homozygotes for Arg151Cys, Arg160Trp, Val60Leu and Val92Met alleles. These strains ranged from darkly pigmented to amelanotic, with all strains of consensus sequence having dark pigmentation. UV sensitivity was found not to be associated with either MC1R genotype or the level of pigmentation with a range of sensitivities seen across all genotypes. Ultrastructural analysis demonstrated that while consensus strains contained stage IV melanosomes in their terminal dendrites, Arg151Cys and Arg160Trp homozygote strains contained only stage II melanosomes. This was despite being able to show expression of tyrosinase and tyrosinase-related protein-1 markers, although at reduced levels and an ability to convert exogenous 3,4-dihydroxyphenyl-alanine (DOPA) to melanin in these strains.

Item Details

Item Type:Refereed Article
Keywords:Pigmentation;Tyrosinase;Melanosome;Melanoma;Pheomelanin
Research Division:Medical and Health Sciences
Research Group:Oncology and Carcinogenesis
Research Field:Cancer Genetics
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Cancer and Related Disorders
Author:Cook, AL (Dr Tony Cook)
ID Code:68211
Year Published:2003
Web of Science® Times Cited:36
Deposited By:Health Sciences A
Deposited On:2011-03-10
Last Modified:2011-03-10
Downloads:0

Repository Staff Only: item control page