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Arabidopsis root K+-efflux conductance activated by hydroxyl radicals: single-channel properties, genetic basis and involvement in stress-induced cell death

Citation

Demidchik, V and Cuin, TA and Svistunenko, D and Smith, SJ and Miller, AJ and Shabala, SN and Sokolik, A and Yurin, V, Arabidopsis root K+-efflux conductance activated by hydroxyl radicals: single-channel properties, genetic basis and involvement in stress-induced cell death, Journal of Cell Science, 123, (9) pp. 1468-1479. ISSN 0021-9533 (2010) [Refereed Article]


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Copyright Statement

Copyright 2010 The Company of Biologists Ltd

Official URL: http://jcs.biologists.org/content/123/9/1468

DOI: doi:10.1242/jcs.064352

Abstract

Reactive oxygen species (ROS) are central to plant stress response, signalling, development and a multitude of other processes. In this study, the plasma-membrane hydroxyl radical (HR)-activated K+ channel responsible for K+ efflux from root cells during stress accompanied by ROS generation is characterised. The channel showed 16-pS unitary conductance and was sensitive to Ca2+, tetraethylammonium, Ba2+, Cs+ and free-radical scavengers. The channel was not found in the gork1-1 mutant, which lacks a major plasma-membrane outwardly rectifying K+ channel. In intact Arabidopsis roots, both HRs and stress induced a dramatic K+ efflux that was much smaller in gork1-1 plants. Tests with electron paramagnetic resonance spectroscopy showed that NaCl can stimulate HR generation in roots and this might lead to K+-channel activation. In animals, activation of K+-efflux channels by HRs can trigger programmed cell death (PCD). PCD symptoms in Arabidopsis roots developed much more slowly in gork1-1 and wild-type plants treated with K+-channel blockers or HR scavengers. Therefore, similar to animal counterparts, plant HR-activated K+ channels are also involved in PCD. Overall, this study provides new insight into the regulation of plant cation transport by ROS and demonstrates possible physiological properties of plant HR-activated K+ channels.

Item Details

Item Type:Refereed Article
Keywords:Ion channels, Plant, Potassium, Programmed cell death, Reactive oxygen species, Stress
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other Plant Production and Plant Primary Products
Objective Field:Plant Production and Plant Primary Products not elsewhere classified
Author:Cuin, TA (Dr Tracey Cuin)
Author:Shabala, SN (Professor Sergey Shabala)
ID Code:68082
Year Published:2010
Web of Science® Times Cited:170
Deposited By:Agricultural Science
Deposited On:2011-03-09
Last Modified:2011-05-12
Downloads:0

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