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Aluminium-induced ion transport in Arabidopsis: the relationship between Al tolerance and root ion flux

Citation

Bose, J and Babourina, O and Shabala, SN and Rengel, Z, Aluminium-induced ion transport in Arabidopsis: the relationship between Al tolerance and root ion flux, Journal of Experimental Botany, 61, (11) pp. 3163-3175. ISSN 0022-0957 (2010) [Refereed Article]

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The definitive publisher-authenticated version is available online at: www.oxfordjournals.org

Official URL: http://www.oxfordjournals.org/

DOI: doi:10.1093/jxb/erq143

Abstract

Aluminium (Al) rhizotoxicity coincides with low pH; however, it is unclear whether plant tolerance to these two factors is controlled by the same mechanism. To address this question, the Al-resistant alr104 mutant, two Al-sensitive mutants (als3 and als5), and wild-type Arabidopsis thaliana were compared in long-term exposure (solution culture) and in short-term exposure experiments (H+ and K+ fluxes, rhizosphere pH, and plasma membrane potential, Em). Based on biomass accumulation, als5 and alr104 showed tolerance to low pH, whereas alr104 was tolerant to the combined low-pH/Al treatment. The sensitivity of the als5 and als3 mutants to the Al stress was similar. The Al-induced decrease in H+ influx at the distal elongation zone (DEZ) and Al-induced H+ efflux at the mature zone (MZ) were higher in the Al-sensitive mutants (als3 and als5) than in the wild type and the alr104 mutant. Under combined low-pH/Al treatment, alr104 and the wild type had depolarized plasma membranes for the entire 30 min measurement period, whereas in the Al-sensitive mutants (als3 and als5), initial depolarization to around 60 mV became hyperpolarization at 110 mV after 20 min. At the DEZ, the Em changes corresponded to the changes in K+ flux: K+ efflux was higher in alr104 and the wild type than in the als3 and als5 mutants. In conclusion, Al tolerance in the alr104 mutant correlated with Em depolarization, higher K+ efflux, and higher H+ influx, which led to a more alkaline rhizosphere under the combined low-pH/Al stress. Low-pH tolerance (als5) was linked to higher H+ uptake under low-pH stress, which was abolished by Al exposure.

Item Details

Item Type:Refereed Article
Keywords:Aluminium toxicity distal root elongation zone H+ flux K+ flux low pH mature root zone plasma membrane potential
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other Plant Production and Plant Primary Products
Objective Field:Plant Production and Plant Primary Products not elsewhere classified
Author:Bose, J (Dr Jayakumar Bose)
Author:Shabala, SN (Professor Sergey Shabala)
ID Code:68067
Year Published:2010
Web of Science® Times Cited:27
Deposited By:Agricultural Science
Deposited On:2011-03-09
Last Modified:2011-04-15
Downloads:0

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