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Competition between uptake of ammonium and potassium in barley and Arabidopsis roots: molecular mechanisms and physiological consequences
Citation
ten Hoopen, F and Cuin, TA and Pedas, P and Hegelund, JN and Shabala, SN and Schjoerring, JK and Jahn, TP, Competition between uptake of ammonium and potassium in barley and Arabidopsis roots: molecular mechanisms and physiological consequences, Journal of Experimental Botany, 61, (9) pp. 2303-2315. ISSN 0022-0957 (2010) [Refereed Article]
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Abstract
Plants can use ammonium (NH4+) as the sole nitrogen source, but at high NH4+ concentrations in the root medium, particularly in combination with a low availability of K+, plants suffer from NH4+ toxicity. To understand the role of K+ transporters and non-selective cation channels in K+/NH4+ interactions better, growth, NH4+ and K+ accumulation and the specific fluxes of NH4+, K+, and H+ were examined in roots of barley (Hordeum vulgare L.) and Arabidopsis seedlings. Net fluxes of K+ and NH4+ were negatively correlated, as were their tissue concentrations, suggesting that there is direct competition during uptake. Pharmacological treatments with the K+ transport inhibitors tetraethyl ammonium (TEA+) and gadolinium (Gd3+) reduced NH4+ influx, and the addition of TEA+ alleviated the NH4+-induced depression of root growth in germinating Arabidopsis plants. Screening of a barley root cDNA library in a yeast mutant lacking all NH4+ and K+ uptake proteins through the deletion of MEP1–3 and TRK1 and TRK2 resulted in the cloning of the barley K+ transporter HvHKT2;1. Further analysis in yeast suggested that HvHKT2;1, AtAKT1, and AtHAK5 transported NH4+, and that K+ supplied at increasing concentrations competed with this NH4+ transport. On the other hand, uptake of K+ by AtHAK5, and to a lesser extent via HvHKT2;1 and AtAKT1, was inhibited by increasing concentrations of NH4+. Together, the results of this study show that plant K+ transporters and channels are able to transport NH4+. Unregulated NH4+ uptake via these transporters may contribute to NH4+ toxicity at low K+ levels, and may explain the alleviation of NH4+ toxicity by K+.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Ammonium toxicity Arabidopsis barley competition gadolinium potassium nutrition tetraethyl ammonium |
| Research Division: | Biological Sciences |
| Research Group: | Plant Biology |
| Research Field: | Plant Physiology |
| Objective Division: | Plant Production and Plant Primary Products |
| Objective Group: | Other Plant Production and Plant Primary Products |
| Objective Field: | Plant Production and Plant Primary Products not elsewhere classified |
| Author: | Cuin, TA (Dr Tracey Cuin) |
| Author: | Shabala, SN (Professor Sergey Shabala) |
| ID Code: | 68050 |
| Year Published: | 2010 |
| Web of Science® Times Cited: | 56 |
| Deposited By: | Agricultural Science |
| Deposited On: | 2011-03-09 |
| Last Modified: | 2011-04-19 |
| Downloads: | 0 |
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