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Unique requirements for reactivation of virus-specific memory B lymphocytes

Citation

Weisel, FJ and Appelt, UK and Schneider, AM and Horlitz, JU and van Rooijen, N and Korner, H and Mach, M and Winkler, TH, Unique requirements for reactivation of virus-specific memory B lymphocytes, Journal of Immunology, 185, (7) pp. 4011-4021. ISSN 0022-1767 (2010) [Refereed Article]


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Copyright Statement

Copyright © 2010 The American Association of Immunologists, Inc. All rights reserved

Official URL: http://www.jimmunol.org/content/185/7/4011

DOI: doi:10.4049/jimmunol.1001540

Abstract

Memory B cells (MBCs) are rapidly activated upon Ag re-exposure in vivo, but the precise requirements for this process are still elusive. To address these requirements, T cell-independent reactivation of MBCs against virus-like particles was analyzed. As few as 25 MBCs are sufficient for a measurable Ab response after adoptive transfer.We found that MBCs were reactivated upon antigenic challenge to normal levels after depletion of macrophages, CD11c+ dendritic cells, and matured follicular dendritic cells. Furthermore, MBC responses were possible in TNF/lymphotoxin a double-deficient mice after partial normalization of lymphoid architecture by means of long-term reconstitution with wild-type bone marrow. Activation did not occur when chimeric mice, which still lack all lymph nodes and Peyer’s patches, were splenectomized prior to MBC transfer. Together with our finding that MBC responses are weak when Ag was administered within minutes after adoptive MBC transfer, these results strongly suggest that MBCs have to occupy specific niches within secondary lymphoid tissue to become fully Ag-responsive. We provide clear evidence that MBCs are not preferentially resident within the splenic marginal zones and show that impaired homing to lymphoid follicles resulted in significantly diminished activation, suggesting that reactivation of MBCs occurred inside lymphoid follicles. Furthermore, comparison of virus-specific MBC T cell-independent reactivation versus primary T cell-independent type II B cell activation revealed unique requirements of MBC activation.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Immunology not elsewhere classified
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Immune System and Allergy
Author:Korner, H (Professor Heinrich Korner)
ID Code:67877
Year Published:2010
Web of Science® Times Cited:15
Deposited By:Menzies Institute for Medical Research
Deposited On:2011-03-08
Last Modified:2011-05-03
Downloads:0

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