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Non-invasive microelectrode potassium flux measurements as a potential tool for early recognition of virus-host compatibility in plants


Shabala, SN and Babourina, OK and Rengel, Z and Nemchinov, LG, Non-invasive microelectrode potassium flux measurements as a potential tool for early recognition of virus-host compatibility in plants, Planta: An International Journal of Plant Biology, 232, (4) pp. 807-815. ISSN 0032-0935 (2010) [Refereed Article]

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DOI: doi:10.1007/s00425-010-1213-y


Diseases caused by plant viruses are widespread, resulting in severe economic losses worldwide. Understanding the cellular basis of defense responses and developing efficient diagnostic tools for early recognition of host specificity to viral infection is, therefore, of great importance. In this work, non-invasive ion selective microelectrodes (the MIFE technique) were used to measure net ion fluxes in mesophyll tissue of host (potato, tomato, tobacco) and non-host (sugar beet and periwinkle) plants in response to infection with Potato virus X (PVX). These results were complemented by FLIM (Fluorescence Lifetime Imaging) measurements of PVX-induced changes in intracellular Ca2+ concentrations. Our results demonstrate that, unlike in other plant-pathogen interactions, Ca2+ signaling appears to be non-essential in recognition of the early stages of viral infection. Instead, we observed significant changes in K+ fluxes as early as 10 min after inoculation. Results of pharmacological experiments and membrane potential measurements pointed out that a significant part of these fluxes may be mediated by depolarization-activated outward-rectifying K+ channels. This may suggest that viral infections trigger a different mechanism of plant defense signaling as compared to signals derived from other microbial pathogens; hence, altered Ca2+ fluxes across the plasma membrane may not be a prerequisite for all elicitor-activated defense reactions. Clearly pronounced host specificity in K+ flux responses suggests that the MIFE technique can be effectively used as a screening tool for the early diagnostics of virus-host compatibility. © 2010 Springer-Verlag.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Field grown vegetable crops
UTAS Author:Shabala, SN (Professor Sergey Shabala)
ID Code:67744
Year Published:2010
Web of Science® Times Cited:13
Deposited By:Agricultural Science
Deposited On:2011-03-07
Last Modified:2011-04-14

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