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The effect of temperature on post-prandial protein synthesis in juvenile barramundi, Lates calcarifer

Citation

Katersky, RS and Carter, CG, The effect of temperature on post-prandial protein synthesis in juvenile barramundi, Lates calcarifer, Comparative Biochemistry and Physiology. Part A: Molecular and Integrative Physiology, 156, (4) pp. 529-536. ISSN 1095-6433 (2010) [Refereed Article]

DOI: doi:10.1016/j.cbpa.2010.04.009

Abstract

The experiment aimed to measure post-prandial protein synthesis at three different temperatures. Juvenile barramundi (10.81 ± 3.46 g) were held at 21, 27 and 33 °C and fed to satiation daily. Samples were taken over a 24 h period at 0 (24 h after the previous meal) and then at 4, 8, 12 and 24 h after feeding to measure protein synthesis in the white muscle, liver and remaining carcass. Protein synthesis at 27 and 33 °C peaked 4 h after feeding in all tissues and returned to pre-feeding rates by 12 h. At 21 °C protein synthesis remained constant over 24 h in all tissues. While the concentration of RNA remained stable over the 24 h cycle and across temperatures, the ribosomal activity increased after feeding. This meant kRNA, not the absolute amount of RNA, was the driving force underlying the post-prandial increase in protein synthesis. However, relative differences in protein synthesis between tissues were attributed to differences in RNA concentration. There was a significant positive relationship between white muscle and whole body protein synthesis. This was the first study to show an interaction between temperature and the time after feeding on protein synthesis for an ectotherm, and that a post-prandial peak in protein synthesis only occurred under optimum temperature conditions. © 2010 Elsevier Inc. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Fin Fish (excl. Tuna)
Author:Katersky, RS (Dr Robin Katersky Barnes)
Author:Carter, CG (Professor Chris Carter)
ID Code:67685
Year Published:2010
Web of Science® Times Cited:8
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2011-03-07
Last Modified:2014-11-24
Downloads:0

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