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Adhesion of Aeromonas sp. to cell lines used as models for intestinal adhesion

Citation

Kirov, SM and Hayward, LJ and Nerrie, MA, Adhesion of Aeromonas sp. to cell lines used as models for intestinal adhesion, Epidemiology and Infection, 115, (3) pp. 465-473. ISSN 0950-2688 (1995) [Refereed Article]

DOI: doi:10.1017/S0950268800058623

Abstract

Adhesion to HEp-2 cells has been shown to correlate with enteropathogenicity for Aeromonas species. Such adhesion is thought to reflect the ability of strains to adhere to human intestinal enterocytes, although HEp-2 cells are not of intestinal origin. In this study strains of Aeromonas veronii biotype sobria isolated from various sources were investigated in parallel assays for their ability to adhere to HEp-8 cells and to an intestinal cell line (Caco-2). Quantitative assays showed identical adhesion values were obtained with both cell lines. Adhesion was best when bacteria were grown at 22°C compared with 37°C and 7°C. Some environmental isolates showed greater adhesion when grown at 7°C than when grown at 37°C. Filamentous structures on these strains are also optimally expressed under the above conditions (reported elsewhere). Mechanical shearing or trypsin treatment to remove surface structures from several adhesive strains grown at 22°C decreased adhesion to cell lines by 50-80% providing further indirect evidence that filamentous adhesins may play a role in cell adhesion for this Aeromonas species.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Medical Microbiology
Research Field:Medical Bacteriology
Objective Division:Health
Objective Group:Other Health
Objective Field:Health not elsewhere classified
Author:Kirov, SM (Associate Professor Sylvia Kirov)
Author:Hayward, LJ (Miss Hayward)
ID Code:6743
Year Published:1995
Web of Science® Times Cited:28
Deposited By:Pathology
Deposited On:1995-08-01
Last Modified:2011-08-25
Downloads:0

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