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Gene expression profiling identifies two regulatory genes controlling dormancy and ABA sensitivity in Arabidopsis seeds

Citation

Barrero, JM and Millar, AA and Griffiths, J and Czechowski, T and Scheible, WR and Udvardi, M and Reid, JB and Ross, JJ and Jacobsen, JV and Gubler, F, Gene expression profiling identifies two regulatory genes controlling dormancy and ABA sensitivity in Arabidopsis seeds, The Plant Journal, 61, (4) pp. 611-622. ISSN 0960-7412 (2010) [Refereed Article]


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Copyright Statement

The definitive published version is available online at: http://onlinelibrary.wiley.com/

Official URL: http://onlinelibrary.wiley.com/

DOI: doi:10.1111/j.1365-313X.2009.04088.x

Abstract

Seed dormancy is a very important trait that maximizes the survival of seed in nature, the control of which can have important repercussions on the yield of many crop species. We have used gene expression profiling to identify genes that are involved in dormancy regulation in Arabidopsis thaliana. RNA was isolated from imbibed dormant (D) and after-ripened (AR) ecotype C24 seeds, and then screened by quantitative RT-PCR (qRT-PCR) for differentially expressed transcription factors (TFs) and other regulatory genes. Out of 2207 genes screened, we have identified 39 that were differentially expressed during the first few hours of imbibition. After analyzing T-DNA insertion mutants for 22 of these genes, two displayed altered dormancy compared with the wild type. These mutants are affected in genes that encode a RING finger and an HDZip protein. The first, named DESPIERTO, is involved in ABA sensitivity during seed development, regulates the expression of ABI3, and produces a complete loss of dormancy when mutated. The second, the HDZip (ATHB20), is expressed during seed germination in the micropylar endosperm and in the root cap, and increases ABA sensitivity and seed dormancy when mutated.

Item Details

Item Type:Refereed Article
Keywords:seed dormancy, germination, ABA, transcription factor profiling, ATHB20.
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural Crops
Objective Field:Horticultural Crops not elsewhere classified
Author:Reid, JB (Professor Jim Reid)
Author:Ross, JJ (Associate Professor John Ross)
ID Code:67358
Year Published:2010
Web of Science® Times Cited:43
Deposited By:Plant Science
Deposited On:2011-03-02
Last Modified:2011-05-12
Downloads:0

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