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Reassessing the role of N-Hydroxytryptamine in auxin biosynthesis
Citation
Tivendale, ND and Davies, NW and Molesworth, PP and Davidson, SE and Smith, JA and Lowe, EK and Reid, JB and Ross, JJ, Reassessing the role of N-Hydroxytryptamine in auxin biosynthesis, Plant Physiology, 154, (4) pp. 1957-1965. ISSN 0032-0889 (2010) [Refereed Article]
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Copyright Statement
Copyright © 2010 by the American Society of Plant Biologists
Official URL: http://www.plantphysiol.org/content/154/4/1957
DOI: doi:10.1104/pp.110.165803
Abstract
The tryptamine pathway is one of five proposed pathways for the biosynthesis of indole-3-acetic acid (IAA), the primary auxin
in plants. The enzymes AtYUC1 (Arabidopsis thaliana), FZY (Solanum lycopersicum), and ZmYUC (Zea mays) are reported to
catalyze the conversion of tryptamine to N-hydroxytryptamine, putatively a rate-limiting step of the tryptamine pathway for
IAA biosynthesis. This conclusion was based on in vitro assays followed by mass spectrometry or HPLC analyses. However,
there are major inconsistencies between the mass spectra reported for the reaction products. Here, we present mass spectral
data for authentic N-hydroxytryptamine, 5-hydroxytryptamine (serotonin), and tryptamine to demonstrate that at least
some of the published mass spectral data for the YUC in vitro product are not consistent with N-hydroxytryptamine. We also
show that tryptamine is not metabolized to IAA in pea (Pisum sativum) seeds, even though a PsYUC-like gene is strongly
expressed in these organs. Combining these findings, we propose that at present there is insufficient evidence to consider
N-hydroxytryptamine an intermediate for IAA biosynthesis.
Item Details
Item Type: | Refereed Article |
---|---|
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Plant Production and Plant Primary Products |
Objective Group: | Horticultural crops |
Objective Field: | Horticultural crops not elsewhere classified |
UTAS Author: | Tivendale, ND (Mr Nathan Tivendale) |
UTAS Author: | Davies, NW (Associate Professor Noel Davies) |
UTAS Author: | Molesworth, PP (Dr Peter Molesworth) |
UTAS Author: | Davidson, SE (Dr Sandra Davidson) |
UTAS Author: | Smith, JA (Associate Professor Jason Smith) |
UTAS Author: | Lowe, EK (Mr Edwin Lowe) |
UTAS Author: | Reid, JB (Professor Jim Reid) |
UTAS Author: | Ross, JJ (Associate Professor John Ross) |
ID Code: | 67348 |
Year Published: | 2010 |
Web of Science® Times Cited: | 50 |
Deposited By: | Plant Science |
Deposited On: | 2011-03-02 |
Last Modified: | 2011-05-12 |
Downloads: | 0 |
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