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Simultaneous estimation of microbial phospholipid fatty acids and diether lipids by capillary gas chromatography

Citation

Virtue, P and Nichols, PD and Boon, PI, Simultaneous estimation of microbial phospholipid fatty acids and diether lipids by capillary gas chromatography, Journal of Microbiological Methods, 25, (2) pp. 177-185. ISSN 0167-7012 (1996) [Refereed Article]

DOI: doi:10.1016/0167-7012(95)00095-X

Abstract

The simultaneous measurement of specific phospholipid ester-linked fatty acids (PLFA) and diether lipids was achieved using high temperature capillary gas chromatography (GC). A GC method was developed and used to simultaneously determine diether lipid and PLFA content in the same GC run. The key features of the method were a slow (10°C/min) temperature ramp through the fatty acid region of the chromatogram followed by a high temperature ramp where the diether lipid eluted. Using this method on both pure bacterial cultures and aquatic sediments, resolution was achieved of the diether lipid and the PLFA, including novel C16 and C18 monounsaturated fatty acids derived from methanotrophs.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Bacteriology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Virtue, P (Associate Professor Patti Virtue)
Author:Nichols, PD (Dr Peter Nichols)
ID Code:6727
Year Published:1996
Web of Science® Times Cited:36
Deposited By:Agricultural Science
Deposited On:1996-08-01
Last Modified:2015-08-26
Downloads:0

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