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Enrichment of the rotifer Brachionus plicatilis fed an Antarctic bacterium containing polyunsaturated fatty acids
Citation
Nichols, DS and Hart, PR and Nichols, PD and McMeekin, TA, Enrichment of the rotifer Brachionus plicatilis fed an Antarctic bacterium containing polyunsaturated fatty acids, Aquaculture, 147, (1-2) pp. 115-125. ISSN 0044-8486 (1996) [Refereed Article]
DOI: doi:10.1016/S0044-8486(96)01391-9
Abstract
The Antarctic bacterium, strain ACAM 456, is known to produce eicosapentaenoic acid (20:5n-3, EPA). Following growth in batch culture, suspensions of this bacterium, at initial concentrations of 107, 108 and 109 cells ml-1, were used as foods for three respective cultures of the rotifer Brachionus plicatilis. At 6 and 24 h. rotifers were removed, harvested and extracted for analysis of fatty acid composition, which was compared to that of rotifers grown on baker's yeast. Incorporation of EPA, along with bacterial fatty acid markets (i13:0, i15:0 and 14:0), was evidenced at all bacterial food concentrations tested. The highest observed incorporation occurred when rotifers were grown in the medium initially containing 109 bacteria ml-1. After 24 h of feeding, the level of EPA reached 9.4% of total fatty acids in the fed rotifers (6.7 ng of EPA rotifer-1). ACAM 456, a bacterial strain with the ability to produce EPA, was therefore shown to be a potential alternative enrichment food for the rotifer Brachionus plicatilis under feeding conditions that may be applicable to many Australian mariculture operations.
Item Details
Item Type: | Refereed Article |
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Research Division: | Agricultural, Veterinary and Food Sciences |
Research Group: | Fisheries sciences |
Research Field: | Aquaculture |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the environmental sciences |
UTAS Author: | Nichols, DS (Dr David Nichols) |
UTAS Author: | Hart, PR (Dr Piers Hart) |
UTAS Author: | Nichols, PD (Dr Peter Nichols) |
UTAS Author: | McMeekin, TA (Professor Thomas McMeekin) |
ID Code: | 6721 |
Year Published: | 1996 |
Web of Science® Times Cited: | 36 |
Deposited By: | Agricultural Science |
Deposited On: | 1996-08-01 |
Last Modified: | 2011-08-16 |
Downloads: | 0 |
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