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TRPV1, NK1 receptor and substance P immunoreactivity and gene expression in the rat lumbosacral spinal cord and urinary bladder after systemic, low dose vanilloid administration
journal contribution
posted on 2023-05-17, 04:22 authored by Heng, YJ, Cassandra SaundersCassandra Saunders, Kunde, DA, Dominic GeraghtyTransient receptor potential vanilloid 1 (TRPV1), neurokinin 1 (NK1) receptor and substance P (SP) immunoreactivity (-ir) and mRNA in the rat lumbosacral spinal cord and urinary bladder were measured 24 hours after s.c. injection of the vanilloids, capsaicin (50 mg/kg) and resiniferatoxin (RTX, 100 ìg/kg), or vehicle (10% ethanol/10% Tween 80/saline). In the spinal cord, capsaicin significantly reduced TRPV1 and SP-ir (40-45%) in laminae I/II compared to controls while RTX produced decreases of ~35%. NK1-ir in the spinal cord was unaffected by both vanilloid treatments. In the bladder, SP-ir was reduced in urothelial cells of some capsaicin- and RTX-treated rats while SP-ir in the suburothelium and muscularis was significantly reduced by RTX. A significant increase in NK1-ir was observed in the urothelium and muscularis after capsaicin administration. Capsaicin significantly increased SP mRNA in the spinal cord, and TRPV1 and SP mRNA in the bladder whereas RTX increased TRPV1, SP and NK1 mRNA in the spinal cord, and TRPV1 and SP mRNA in the bladder. These data suggest that stimulation of TRPV1 by low dose vanilloid administration can rapidly (within 24 hours) alter both transcription and translation of TRPV1 channels, SP and NK1 receptors in the rat urinary bladder and spinal cord.
History
Publication title
Regulatory PeptidesVolume
167Issue
2-3Pagination
250-258ISSN
0167-0115Department/School
School of Health SciencesPublisher
Elsevier B.V.Place of publication
AmsterdamRights statement
The definitive version is available at http://www.sciencedirect.comRepository Status
- Restricted