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TRPV1, NK1 receptor and substance P immunoreactivity and gene expression in the rat lumbosacral spinal cord and urinary bladder after systemic, low dose vanilloid administration

Citation

Heng, YJ and Saunders, CIM and Kunde, DA and Geraghty, DP, TRPV1, NK1 receptor and substance P immunoreactivity and gene expression in the rat lumbosacral spinal cord and urinary bladder after systemic, low dose vanilloid administration, Regulatory Peptides, 167, (2-3) pp. 250-258. ISSN 0167-0115 (2011) [Refereed Article]


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DOI: doi:10.1016/j.regpep.2011.02.004

Abstract

Transient receptor potential vanilloid 1 (TRPV1), neurokinin 1 (NK1) receptor and substance P (SP) immunoreactivity (-ir) and mRNA in the rat lumbosacral spinal cord and urinary bladder were measured 24 hours after s.c. injection of the vanilloids, capsaicin (50 mg/kg) and resiniferatoxin (RTX, 100 ėg/kg), or vehicle (10% ethanol/10% Tween 80/saline). In the spinal cord, capsaicin significantly reduced TRPV1 and SP-ir (40-45%) in laminae I/II compared to controls while RTX produced decreases of ~35%. NK1-ir in the spinal cord was unaffected by both vanilloid treatments. In the bladder, SP-ir was reduced in urothelial cells of some capsaicin- and RTX-treated rats while SP-ir in the suburothelium and muscularis was significantly reduced by RTX. A significant increase in NK1-ir was observed in the urothelium and muscularis after capsaicin administration. Capsaicin significantly increased SP mRNA in the spinal cord, and TRPV1 and SP mRNA in the bladder whereas RTX increased TRPV1, SP and NK1 mRNA in the spinal cord, and TRPV1 and SP mRNA in the bladder. These data suggest that stimulation of TRPV1 by low dose vanilloid administration can rapidly (within 24 hours) alter both transcription and translation of TRPV1 channels, SP and NK1 receptors in the rat urinary bladder and spinal cord.

Item Details

Item Type:Refereed Article
Keywords:Vanilloid, respiration, tachykinin, autoradiography, substance P, resiniferatoxin, TRPV1
Research Division:Medical and Health Sciences
Research Group:Pharmacology and Pharmaceutical Sciences
Research Field:Basic Pharmacology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Urogenital System and Disorders
Author:Heng, YJ (Miss Yu Heng)
Author:Saunders, CIM (Dr Cassie Saunders)
Author:Kunde, DA (Dr Dale Kunde)
Author:Geraghty, DP (Professor Dominic Geraghty)
ID Code:66704
Year Published:2011
Web of Science® Times Cited:3
Deposited By:Health Sciences A
Deposited On:2011-02-09
Last Modified:2012-04-18
Downloads:2 View Download Statistics

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