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Metaproteogenomic analysis of a dominant green sulfur bacterium from Ace Lake, Antarctica

Citation

Ng, C and DeMaere, MZ and Williams, TJ and Lauro, FM and Raftery, M and Gibson, JAE and Andrews-Pfannkoch, C and Lewis, M and Hoffman, JM and Thomas, T and Cavicchioli, R, Metaproteogenomic analysis of a dominant green sulfur bacterium from Ace Lake, Antarctica, ISME Journal, 4, (8) pp. 1002-1019. ISSN 1751-7362 (2010) [Refereed Article]


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Copyright © 2010 Nature Publishing Group

Official URL: http://www.nature.com/ismej/journal/v4/n8/full/ism...

DOI: doi:10.1038/ismej.2010.28

Abstract

Green sulfur bacteria (GSB) (Chlorobiaceae) are primary producers that are important in global carbon and sulfur cycling in natural environments. An almost complete genome sequence for a single, dominant GSB species (‘C-Ace’) was assembled from shotgun sequence data of an environmental sample taken from the O2–H2S interface of the water column of Ace Lake, Antarctica. Approximately 34Mb of DNA sequence data were assembled into nine scaffolds totaling 1.79 Mb, representing approximately 19-fold coverage for the C-Ace composite genome. A high level (B31%)of metaproteomic coverage was achieved using matched biomass. The metaproteogenomic approach provided unique insight into the protein complement required for dominating the microbial community under cold, nutrient-limited, oxygen-limited and extremely varied annual light conditions. C-Ace shows physiological traits that promote its ability to compete very effectively with other GSB and gain dominance (for example, specific bacteriochlorophylls, mechanisms of cold adaptation) as well as a syntrophic relationship with sulfate-reducing bacteria that provides a mechanism for the exchange of sulfur compounds. As a result we are able to propose an explanation of the active biological processes promoted by cold-adapted GSB and the adaptive strategies they use to thrive under the severe physiochemical conditions prevailing in polar environments.

Item Details

Item Type:Refereed Article
Keywords:green sulfur bacterium; metagenomics; metaproteomics; cold adaptation; Antarctic
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbial Ecology
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Antarctic and Sub-Antarctic Flora, Fauna and Biodiversity
Author:Gibson, JAE (Dr John Gibson)
ID Code:66533
Year Published:2010
Web of Science® Times Cited:52
Deposited By:TAFI - Marine Research Laboratory
Deposited On:2011-02-01
Last Modified:2015-02-02
Downloads:0

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