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Metaproteogenomic analysis of a dominant green sulfur bacterium from Ace Lake, Antarctica
Citation
Ng, C and DeMaere, MZ and Williams, TJ and Lauro, FM and Raftery, M and Gibson, JAE and Andrews-Pfannkoch, C and Lewis, M and Hoffman, JM and Thomas, T and Cavicchioli, R, Metaproteogenomic analysis of a dominant green sulfur bacterium from Ace Lake, Antarctica, ISME Journal, 4, (8) pp. 1002-1019. ISSN 1751-7362 (2010) [Refereed Article]
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Copyright Statement
Copyright © 2010 Nature Publishing Group
Official URL: http://www.nature.com/ismej/journal/v4/n8/full/ism...
DOI: doi:10.1038/ismej.2010.28
Abstract
Green sulfur bacteria (GSB) (Chlorobiaceae) are primary producers that are important in global carbon and sulfur cycling in natural environments. An almost complete genome sequence for a single, dominant GSB species (‘C-Ace’) was assembled from shotgun sequence data of an environmental sample taken from the O2–H2S interface of the water column of Ace Lake, Antarctica.
Approximately 34Mb of DNA sequence data were assembled into nine scaffolds totaling 1.79 Mb, representing approximately 19-fold coverage for the C-Ace composite genome. A high level (B31%)of metaproteomic coverage was achieved using matched biomass. The metaproteogenomic approach provided unique insight into the protein complement required for dominating the microbial community under cold, nutrient-limited, oxygen-limited and extremely varied annual light
conditions. C-Ace shows physiological traits that promote its ability to compete very effectively with other GSB and gain dominance (for example, specific bacteriochlorophylls, mechanisms of cold adaptation) as well as a syntrophic relationship with sulfate-reducing bacteria that provides a
mechanism for the exchange of sulfur compounds. As a result we are able to propose an explanation of the active biological processes promoted by cold-adapted GSB and the adaptive strategies they use to thrive under the severe physiochemical conditions prevailing in polar environments.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | green sulfur bacterium; metagenomics; metaproteomics; cold adaptation; Antarctic |
| Research Division: | Biological Sciences |
| Research Group: | Microbiology |
| Research Field: | Microbial ecology |
| Objective Division: | Environmental Management |
| Objective Group: | Management of Antarctic and Southern Ocean environments |
| Objective Field: | Biodiversity in Antarctic and Southern Ocean environments |
| UTAS Author: | Gibson, JAE (Dr John Gibson) |
| ID Code: | 66533 |
| Year Published: | 2010 |
| Web of Science® Times Cited: | 79 |
| Deposited By: | TAFI - Marine Research Laboratory |
| Deposited On: | 2011-02-01 |
| Last Modified: | 2015-02-02 |
| Downloads: | 0 |
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