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Comparative assessment of genetic and epigenetic variation among regenerants of potato (Solanum tuberosum) derived from long-term nodal tissue-culture and cell selection

Citation

Dann, AL and Wilson, CR, Comparative assessment of genetic and epigenetic variation among regenerants of potato (Solanum tuberosum) derived from long-term nodal tissue-culture and cell selection, Plant Cell Reports, 30, (4) pp. 631-639. ISSN 0721-7714 (2011) [Refereed Article]

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Copyright Statement

The final publication is available at http://www.springerlink.com

Official URL: http://www.springerlink.com

DOI: doi:10.1007/s00299-010-0983-9

Abstract

Three long-term nodal tissued cultured Russet Burbank potato clones and nine thaxtomin A-treated regenerant lines, derived from the nodal lines, were assessed for genetic and epigenetic (in the form of DNA methylation) differences by AFLP and MSAP. The treated regenerant lines were originally selected for superior resistance to common scab disease and acceptable tuber yield in pot and field trials. The long-term, tissue culture clone lines exhibited genetic (8.7515.63% polymorphisms) and epigenetic (12.5626.13% polymorphisms) differences between them and may represent a stress response induced by normal plant growth disruption. The thaxtomin A-treated regenerant lines exhibited much higher significant (p < 0.05) genetic (229.38%) and epigenetic (45.2251.76%) polymorphisms than the nodal cultured parent clones. Methylation-sensitive mutations accumulated within the regenerant lines are significantly correlated (p < 0.05) to disease resistance. However, linking phenotypic differences that could be of benefit to potato growers, to single gene sequence polymorphisms in a tetraploid plant such as the potato would be extremely difficult since it is assumed many desirable traits are under polygenic control.

Item Details

Item Type:Refereed Article
Keywords:tissue culture-induced variation,  AFLP, cytosine methylation,  MSAP, disease resistance, thaxtomin A
Research Division:Agricultural and Veterinary Sciences
Research Group:Horticultural Production
Research Field:Horticultural Crop Improvement (Selection and Breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural Crops
Objective Field:Vegetables
Author:Dann, AL (Dr Alison Dann)
Author:Wilson, CR (Associate Professor Calum Wilson)
ID Code:66136
Year Published:2011
Web of Science® Times Cited:24
Deposited By:Agricultural Science
Deposited On:2010-12-23
Last Modified:2013-09-16
Downloads:4 View Download Statistics

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