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A murine xenograft model for a transmissible cancer in Tasmanian devils

Citation

Kreiss, A and Tovar, C and Obendorf, DL and Dun, K and Woods, GM, A murine xenograft model for a transmissible cancer in Tasmanian devils, Veterinary Pathology, 48, (2) pp. 475-481. ISSN 0300-9858 (2010) [Refereed Article]


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Copyright Statement

Copyright 2010 SAGE Publications

Official URL: http://www.uk.sagepub.com/

DOI: doi:10.1177/0300985810380398

Abstract

The number of Tasmanian devils in the wild is rapidly declining owing to a transmissible cancer, devil facial tumor disease (DFTD). Although progress has been made to understand the spread of this disease, crucial research on the pathogenesis of DFTD has been limited because of the threatened status of the host species. Here, the authors describe the development of a NOD/SCID (nonobese diabetic / severe combined immunodeficiency) mouse model that reproduces DFTD and provides a much-needed model to undertake studies into this intriguing transmissible cancer. Histologically, the DFTD produced in NOD/SCID mice (xenografted DFTD) was indistinguishable from the DFTD identified in Tasmanian devils. At the protein level, all xenografted DFTD tumors expressed periaxin, a marker that confirmed the diagnosis of DFTD. The karyotype of DFTD in NOD/SCID mice reproduced similar chromosomal alterations as seen in diseased devils. Furthermore, each NOD/SCID mouse inoculated with cultured DFTD tumor cells developed tumors, whereas DFTD did not develop in any of the inoculated immune-competent BALB/c mice.

Item Details

Item Type:Refereed Article
Keywords:Tasmanian devil, devil facial tumor disease, NOD/SCID mouse, cytogenetic
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Tumour Immunology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Cancer and Related Disorders
Author:Kreiss, A (Dr Alexandre Kreiss)
Author:Tovar, C (Dr Cesar Tovar Lopez)
Author:Obendorf, DL (Dr David Obendorf)
Author:Woods, GM (Professor Gregory Woods)
ID Code:66086
Year Published:2010
Web of Science® Times Cited:8
Deposited By:Menzies Institute for Medical Research
Deposited On:2010-12-21
Last Modified:2011-05-02
Downloads:2 View Download Statistics

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