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A murine xenograft model for a transmissible cancer in Tasmanian devils
journal contribution
posted on 2023-05-17, 04:06 authored by Kreiss, A, Cesar Tovar LopezCesar Tovar Lopez, Obendorf, DL, Dun, K, Gregory WoodsGregory WoodsThe number of Tasmanian devils in the wild is rapidly declining owing to a transmissible cancer, devil facial tumor disease (DFTD). Although progress has been made to understand the spread of this disease, crucial research on the pathogenesis of DFTD has been limited because of the threatened status of the host species. Here, the authors describe the development of a NOD/SCID (nonobese diabetic / severe combined immunodeficiency) mouse model that reproduces DFTD and provides a much-needed model to undertake studies into this intriguing transmissible cancer. Histologically, the DFTD produced in NOD/SCID mice (xenografted DFTD) was indistinguishable from the DFTD identified in Tasmanian devils. At the protein level, all xenografted DFTD tumors expressed periaxin, a marker that confirmed the diagnosis of DFTD. The karyotype of DFTD in NOD/SCID mice reproduced similar chromosomal alterations as seen in diseased devils. Furthermore, each NOD/SCID mouse inoculated with cultured DFTD tumor cells developed tumors, whereas DFTD did not develop in any of the inoculated immune-competent BALB/c mice.
History
Publication title
Veterinary PathologyVolume
48Pagination
475-481ISSN
0300-9858Department/School
Menzies Institute for Medical ResearchPublisher
Amer Coll Vet PathologistPlace of publication
810 East 10Th Street, Lawrence, USA, Ks, 66044Rights statement
Copyright © 2010 SAGE PublicationsRepository Status
- Restricted