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AHSP (α-haemoglobin-stabilizing protein) stabilizes apo-α-haemoglobin in a partially folded state

Citation

Kumar, KK and Dickson, CF and Weiss, MJ and Mackay, JP and Gell, DA, AHSP (α-haemoglobin-stabilizing protein) stabilizes apo-α-haemoglobin in a partially folded state, Biochemical Journal, 432, (2) pp. 275-282. ISSN 0264-6021 (2010) [Refereed Article]


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Copyright Statement

© The Authors Journal compilation © 2010 Portland Press

Official URL: http://www.biochemj.org/bj/432/bj4320275.htm

DOI: doi:10.1042/BJ20100642

Abstract

To produce functional Hb (haemoglobin), nascent !-globin (!o) and "-globin ("o) chains must each bind a single haem molecule (to form !h and "h) and interact together to form heterodimers.The precise sequence of binding events is unknown, and it has been suggested that additional factors might enhance the efficiency of Hb folding. AHSP (!-haemoglobin-stabilizing protein) has been shown previously to bind !h and regulate redox activity of the haem iron. In the present study, we used a combination of classical and dynamic light scattering and NMR spectroscopy to demonstrate that AHSP forms a heterodimeric complex with !o that inhibits !o aggregation and promotes !o folding in the absence of haem. These findings indicate that AHSP may function as an !o-specific chaperone, and suggest an important role for !o in guiding Hb assembly by stabilizing "o and inhibiting off-pathway self-association of "h.

Item Details

Item Type:Refereed Article
Keywords:a-haemoglobin-stabilizing protein (AHSP), aggregation, apo-a-haemoglobin, chaperone
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Structural Biology (incl. Macromolecular Modelling)
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Dickson, CF (Miss Claire Dickson)
Author:Gell, DA (Dr David Gell)
ID Code:66023
Year Published:2010
Web of Science® Times Cited:8
Deposited By:Menzies Institute for Medical Research
Deposited On:2010-12-15
Last Modified:2011-05-02
Downloads:0

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