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AHSP (α-haemoglobin-stabilizing protein) stabilizes apo-α-haemoglobin in a partially folded state
Citation
Kumar, KK and Dickson, CF and Weiss, MJ and Mackay, JP and Gell, DA, AHSP (α-haemoglobin-stabilizing protein) stabilizes apo-α-haemoglobin in a partially folded state, Biochemical Journal, 432, (2) pp. 275-282. ISSN 0264-6021 (2010) [Refereed Article]
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Copyright Statement
© The Authors Journal compilation © 2010 Portland Press
Official URL: http://www.biochemj.org/bj/432/bj4320275.htm
Abstract
To produce functional Hb (haemoglobin), nascent !-globin (!o)
and "-globin ("o) chains must each bind a single haem molecule
(to form !h and "h) and interact together to form heterodimers.The
precise sequence of binding events is unknown, and it has been
suggested that additional factors might enhance the efficiency
of Hb folding. AHSP (!-haemoglobin-stabilizing protein) has
been shown previously to bind !h and regulate redox activity
of the haem iron. In the present study, we used a combination of
classical and dynamic light scattering and NMR spectroscopy to
demonstrate that AHSP forms a heterodimeric complex with !o
that inhibits !o aggregation and promotes !o folding in the absence
of haem. These findings indicate that AHSP may function as an
!o-specific chaperone, and suggest an important role for !o in
guiding Hb assembly by stabilizing "o and inhibiting off-pathway
self-association of "h.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | a-haemoglobin-stabilizing protein (AHSP), aggregation, apo-a-haemoglobin, chaperone |
Research Division: | Biological Sciences |
Research Group: | Biochemistry and cell biology |
Research Field: | Structural biology (incl. macromolecular modelling) |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Dickson, CF (Miss Claire Dickson) |
UTAS Author: | Gell, DA (Dr David Gell) |
ID Code: | 66023 |
Year Published: | 2010 |
Web of Science® Times Cited: | 13 |
Deposited By: | Menzies Institute for Medical Research |
Deposited On: | 2010-12-15 |
Last Modified: | 2011-05-02 |
Downloads: | 0 |
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