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Immunological aspects of amoebic gill disease in salmonids


Akhlaghi, M and Munday, BL and Rough, KM and Whittington, RJ, Immunological aspects of amoebic gill disease in salmonids, Diseases of Aquatic Organisms, 25, (1-2) pp. 23-31. ISSN 0177-5103 (1996) [Refereed Article]

DOI: doi:10.3354/dao025023


Paramoeba sp. antigens emulsified with Freund's complete and incomplete adjuvants were immunogenic in rainbow trout Oncorhynchus mykiss and Atlantic salmon Salmo salar by intraperitoneal (i.p.) injection as well as for sheep and rabbit by subcutaneous and intravenous injections respectively. Atlantic salmon were immunized passively with an i.p injection (0.1 ml 100 g-1 fish body weight) of sheep anti-Paramoeba sp. antibodies (APA). Sheep APA were detected in fish sera by enzyme-linked immunosorbent assay for up to 8 wk after i.p. injection. Assessments of passive and active immunization were undertaken concurrently in order to demonstrate their relative efficacies and especially to evaluate the practical potential of passive immunization. Immunized fish were exposed to natural infection by cohabitation with infected Atlantic salmon 45 d post-immunization. Transmission of the disease was successful; however, unequivocal protection was not demonstrated in any of the immunized fish suggesting a minor role for systemic antibodies in protection against amoebic gill disease,.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbiology not elsewhere classified
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - wild caught
Objective Field:Fisheries - wild caught not elsewhere classified
UTAS Author:Akhlaghi, M (Mr Mostafa Akhlaghi)
UTAS Author:Munday, BL (Dr Barry Munday)
UTAS Author:Rough, KM (Ms Kirsten May Rough)
ID Code:6601
Year Published:1996
Web of Science® Times Cited:24
Deposited By:TAFI - Aquaculture
Deposited On:1996-08-01
Last Modified:2011-08-16

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