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Monitoring associations between clade-level variation, overall community structure and ecosystem function in enhanced biological phosphorus removal (EBPR) systems using terminal-restriction fragment length polymorphism (T-RFLP)
Citation
Slater, FR and Johnson, CR and Blackall, LL and Beiko, RG and Bond, PL, Monitoring associations between clade-level variation, overall community structure and ecosystem function in enhanced biological phosphorus removal (EBPR) systems using terminal-restriction fragment length polymorphism (T-RFLP), Water Research, 44, (17) pp. 4908-4923. ISSN 0043-1354 (2010) [Refereed Article]
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Copyright Statement
The definitive version is available at http://www.sciencedirect.com
Official URL: http://www.sciencedirect.com
DOI: doi:10.1016/j.watres.2010.07.028
Abstract
The role of Candidatus "Accumulibacter phosphatis" (Accumulibacter) in enhanced bio-
logical phosphorus removal (EBPR) is well established but the relevance of different
Accumulibacter clades to the performance of EBPR systems is unknown. We developed
a terminal-restriction fragment length polymorphism (T-RFLP) technique to monitor
changes in the relative abundance of key members of the bacterial community, including
Accumulibacter clades, in four replicate mini-sequencing batch reactors (mSBRs) operated
for EBPR over a 35-day period. The ability of the T-RFLP technique to detect trends was
confirmed using fluorescence in situ hybridisation (FISH). EBPR performance varied
between reactors and over time; by day 35, performance was maintained in mSBR2 whilst
it had deteriorated in mSBR1. However, reproducible trends in structureefunction rela-
tionships were detected in the mSBRs. EBPR performance was strongly associated with the
relative abundance of total Accumulibacter. A shift in the ratio of the dominant Accu-
mulibacter clades was also detected, with Type IA associated with good EBPR performance
and Type IIC associated with poor EBPR performance. Changes in ecosystem function of
the mSBRs in the early stages of the experiment were more closely associated with changes
in the abundance of (unknown) members of the flanking community than of either
Accumulibacter or Candidatus "Competibacter phosphatis". This study therefore reveals
a hitherto unrecorded and complex relationship between Accumulibacter clades, the
flanking community and ecosystem function of laboratory-scale EBPR systems.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Biological Sciences |
| Research Group: | Biochemistry and cell biology |
| Research Field: | Structural biology (incl. macromolecular modelling) |
| Objective Division: | Environmental Management |
| Objective Group: | Other environmental management |
| Objective Field: | Other environmental management not elsewhere classified |
| UTAS Author: | Johnson, CR (Professor Craig Johnson) |
| ID Code: | 65929 |
| Year Published: | 2010 |
| Web of Science® Times Cited: | 46 |
| Deposited By: | Zoology |
| Deposited On: | 2010-12-09 |
| Last Modified: | 2011-04-08 |
| Downloads: | 1 View Download Statistics |
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