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White LEDs as broad spectrum light sources for spectrophotometry: Demonstration in the visible spectrum range in a diode-array spectrophotometric detector
Citation
Piasecki, T and Breadmore, MC and Macka, M, White LEDs as broad spectrum light sources for spectrophotometry: Demonstration in the visible spectrum range in a diode-array spectrophotometric detector, Electrophoresis, 31, (22) pp. 3737-3744. ISSN 0173-0835 (2010) [Refereed Article]
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Copyright Statement
The definitive published version is available online at: http://onlinelibrary.wiley.com/
Official URL: http://onlinelibrary.wiley.com/
DOI: doi:10.1002/elps.201000341
Abstract
Although traditional lamps, such as deuterium lamps, are suitable for bench-top instrumentation, their compatibility with the requirements of modern miniaturized instrumentation is limited. This study investigates the option of utilizing solid-state light source technology, namely white LEDs, as a broad band spectrum source for spectrophotometry. Several white light LEDs of both RGB and white phosphorus have been characterized in terms of their emission spectra and energy output and a white phosphorus Luxeon LED was then chosen for demonstration as a light source for visible-spectrum spectrophotometry conducted in CE. The Luxeon LED was fixed onto the base of a dismounted deuterium (D2) lamp so that the light-emitting spot was geometrically positioned exactly where the light-emitting spot of the original D2 lamp is placed. In this manner, the detector of a commercial CE instrument equipped with a DAD was not modified in any way. As the detector hardware and electronics remained the same, the change of the deuterium lamp for the Luxeon white LED allowed a direct comparison of their performances. Several anionic dyes as model analytes with absorption maxima between 450 and 600 nm were separated by CE in an electrolyte of 0.01 mol/L sodium tetraborate. The absorbance baseline noise as the key parameter was 5× lower for the white LED lamp, showing clearly superior performance to the deuterium lamp in the available, i.e. visible part of the spectrum.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | * Capillary separation methods; * CE; * Miniaturization; * Spectrophotometry; * White LED |
| Research Division: | Chemical Sciences |
| Research Group: | Analytical Chemistry |
| Research Field: | Separation Science |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Chemical Sciences |
| UTAS Author: | Breadmore, MC (Professor Michael Breadmore) |
| UTAS Author: | Macka, M (Professor Mirek Macka) |
| ID Code: | 65642 |
| Year Published: | 2010 |
| Funding Support: | Australian Research Council (DP0984745) |
| Web of Science® Times Cited: | 6 |
| Deposited By: | Austn Centre for Research in Separation Science |
| Deposited On: | 2010-11-29 |
| Last Modified: | 2011-05-03 |
| Downloads: | 0 |
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