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White LEDs as broad spectrum light sources for spectrophotometry: Demonstration in the visible spectrum range in a diode-array spectrophotometric detector

Citation

Piasecki, T and Breadmore, MC and Macka, M, White LEDs as broad spectrum light sources for spectrophotometry: Demonstration in the visible spectrum range in a diode-array spectrophotometric detector, Electrophoresis, 31, (22) pp. 3737-3744. ISSN 0173-0835 (2010) [Refereed Article]


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The definitive published version is available online at: http://onlinelibrary.wiley.com/

Official URL: http://onlinelibrary.wiley.com/

DOI: doi:10.1002/elps.201000341

Abstract

Although traditional lamps, such as deuterium lamps, are suitable for bench-top instrumentation, their compatibility with the requirements of modern miniaturized instrumentation is limited. This study investigates the option of utilizing solid-state light source technology, namely white LEDs, as a broad band spectrum source for spectrophotometry. Several white light LEDs of both RGB and white phosphorus have been characterized in terms of their emission spectra and energy output and a white phosphorus Luxeon LED was then chosen for demonstration as a light source for visible-spectrum spectrophotometry conducted in CE. The Luxeon LED was fixed onto the base of a dismounted deuterium (D2) lamp so that the light-emitting spot was geometrically positioned exactly where the light-emitting spot of the original D2 lamp is placed. In this manner, the detector of a commercial CE instrument equipped with a DAD was not modified in any way. As the detector hardware and electronics remained the same, the change of the deuterium lamp for the Luxeon white LED allowed a direct comparison of their performances. Several anionic dyes as model analytes with absorption maxima between 450 and 600 nm were separated by CE in an electrolyte of 0.01 mol/L sodium tetraborate. The absorbance baseline noise as the key parameter was 5 lower for the white LED lamp, showing clearly superior performance to the deuterium lamp in the available, i.e. visible part of the spectrum.

Item Details

Item Type:Refereed Article
Keywords: * Capillary separation methods; * CE; * Miniaturization; * Spectrophotometry; * White LED
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Breadmore, MC (Professor Michael Breadmore)
Author:Macka, M (Professor Mirek Macka)
ID Code:65642
Year Published:2010
Funding Support:Australian Research Council (DP0984745)
Web of Science® Times Cited:5
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2010-11-29
Last Modified:2011-05-03
Downloads:0

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