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In vitro antioxidant, antiplatelet and anti-inflammatory activity of Carpobrotus rossii (pigface) extract

Citation

Geraghty, DP and Ahuja, KDK and Pittaway, J and Shing, C and Jacobson, GA and Jager, N and Jurkovic, S and Narkowicz, C and Saunders, CI and Ball, M and Pinkard, A and Vennavaram, RR and Adams, MJ, In vitro antioxidant, antiplatelet and anti-inflammatory activity of Carpobrotus rossii (pigface) extract, Journal of Ethnopharmacology, 134, (1) pp. 97-103. ISSN 0378-8741 (2011) [Refereed Article]


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DOI: doi:10.1016/j.jep.2010.11.060

Abstract

Ethnopharmacological relevance Carpobrotus rossii (CR) has a history of use as a food and therapeutic agent by Australian indigenous peoples and early European settlers and is believed to contain a number of pharmacologically active polyphenolic compounds. Aims of the study Oxidation of low density lipoprotein (LDL), platelet aggregation, and inflammation contribute to the development and progression of atherosclerosis. The aim of the present study was to investigate the antioxidant, antiplatelet and anti-inflammatory activity of CR extract using human blood components. Materials and methods An assay employing in vitro copper-induced oxidation of serum lipids was used to assess antioxidant activity of CR extract (and tannin, flavonoid and pre- and post-flavonoid fractions). The effects of CR extract on ADP- and collagen-induced platelet aggregation, and on basal (unstimulated) and lipopolysaccharide (LPS)- and phytohaemagglutinin A (PHA)-stimulated cytokine release from peripheral blood mononuclear cells (PBMC) were also investigated. Results CR extract increased the lag time of serum oxidation (maximum of ~ 4-fold at 20 g/ml) in a concentration-dependent manner. The antioxidant activity resided only in the tannin and post-flavonoid fractions. CR had no effect on ADP-induced platelet aggregation, but significantly decreased collagen-induced platelet aggregation. LPS, but not PHA, significantly increased the release of IL-1 and TNF- from PBMC. CR extract alone inhibited monocyte chemoattractant protein (MCP)-1 release and in the presence of LPS, inhibited IL-10, TNF and MCP-1 release compared to LPS alone. Conclusion CR has significant in vitro antioxidant, antiplatelet and, potentially, anti-inflammatory activity. Keywords: Carpobrotus rossii; antioxidant; lipid oxidation; platelet aggregation; cytokine release

Item Details

Item Type:Refereed Article
Keywords:Carpobrotus rossii; antioxidant; lipid oxidation; platelet aggregation; cytokine release
Research Division:Medical and Health Sciences
Research Group:Pharmacology and Pharmaceutical Sciences
Research Field:Basic Pharmacology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Cardiovascular System and Diseases
Author:Geraghty, DP (Professor Dominic Geraghty)
Author:Ahuja, KDK (Dr Kiran Ahuja)
Author:Pittaway, J (Ms Jane Pittaway)
Author:Shing, C (Dr Cecilia Shing)
Author:Jacobson, GA (Dr Glenn Jacobson)
Author:Jager, N (Ms Nynke Jager)
Author:Jurkovic, S (Mr Sasa Jurkovic)
Author:Narkowicz, C (Dr Christian Narkowicz)
Author:Saunders, CI (Dr Cassie Saunders)
Author:Ball, M (Professor Madeleine Ball)
Author:Pinkard, A (Miss Alexandra Pinkard)
Author:Vennavaram, RR (Mr Raghu Reddy Vennavaram)
Author:Adams, MJ (Dr Murray Adams)
ID Code:65627
Year Published:2011
Web of Science® Times Cited:5
Deposited By:Health Sciences A
Deposited On:2010-11-26
Last Modified:2012-04-18
Downloads:5 View Download Statistics

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