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Physical characterisation of Tenacibaculum maritimum for vaccine development


van Gelderen, R and Carson, J and Gudkovs, N and Nowak, B, Physical characterisation of Tenacibaculum maritimum for vaccine development, Journal of Applied Microbiology, 109, (5) pp. 1668-1676. ISSN 1364-5072 (2010) [Refereed Article]

Copyright Statement

Copyright 2010 The Authors, Journal of Applied Microbiology, The Society for Applied Microbiology

DOI: doi:10.1111/j.1365-2672.2010.04795.x


Aims: Tenacibaculum maritimum is a well known fish pathogen worldwide, affecting many fish species including Atlantic salmon in Tasmania, Australia. The aim of this study was to characterise and understand the similarities and differences between the isolates in order to select isolates for later pathogenicity and vaccination trials.

Methods and Results: Several physical characterisation tests were carried out: whole cell protein profiles, lipopolysaccharide profiles (LPS), extracellular product profiles (ECP), indirect immunofluorescent antibody test (IFAT) and hydrophobicity. The Tasmanian strains of T. maritimum appear relatively homogeneous physically, but antigenically different. All isolates were hydrophobic and produce a variety of ECP profiles. There were two isolates that stand out in all assays (89/4747 and 01/0356-7) and showed great variation from the other isolates. All isolates have been confirmed as T. maritimum.

Conclusions: Based on the tests carried out three isolates were chosen for in vivo trials: 89/4747, 89/4762, 00/3280.

Significance and Impact of the Study: This is the first study to characterise T. maritimum isolates from Tasmanian waters. The opportunity to develop vaccines for the Tasmanian salmonid aquaculture industry is enhanced by a greater understanding of the physical characteristics of pathogens.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Fish pests and diseases
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Aquaculture fin fish (excl. tuna)
UTAS Author:van Gelderen, R (Ms Rebecca van Gelderen)
UTAS Author:Carson, J (Dr Jeremy Carson)
UTAS Author:Nowak, B (Professor Barbara Nowak)
ID Code:65529
Year Published:2010
Web of Science® Times Cited:7
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2010-11-22
Last Modified:2015-11-04

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