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MicroRNAs miR-17 and miR-20a Inhibit T Cell Activation Genes and Are Under-Expressed in MS Whole Blood

Citation

Cox, MB and Cairns, MJ and Gandhi, KS and Carroll, AP and Moscovis, S and Stewart, GJ and Broadley, S and Scott, RJ and Booth, DR and Lechner-Scott, J and Bahlo, M and Butzkueven, H and Brown, MA and Foote, SJ and Griffiths, L and Kilpatrick, TJ and Moscato, P and Perreau, VM and Rubio, JP and Stankovich, J and Taylor, BV and Wiley, J and Heard, RN, MicroRNAs miR-17 and miR-20a Inhibit T Cell Activation Genes and Are Under-Expressed in MS Whole Blood, P L o S One, 5, (8) EJ ISSN 1932-6203 (2010) [Refereed Article]


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Copyright Statement

Copyright © 2010 Cox et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Official URL: http://plosone.org

DOI: doi:10.1371/journal.pone.0012132

Abstract

It is well established that Multiple Sclerosis (MS) is an immune mediated disease. Little is known about what drives the differential control of the immune system in MS patients compared to unaffected individuals. MicroRNAs (miRNAs) are small non-coding nucleic acids that are involved in the control of gene expression. Their potential role in T cell activation and neurodegenerative disease has recently been recognised and they are therefore excellent candidates for further studies in MS. We investigated the transcriptome of currently known miRNAs using miRNA microarray analysis in peripheral blood samples of 59 treatment naý¨ve MS patients and 37 controls. Of these 59, 18 had a primary progressive, 17 a secondary progressive and 24 a relapsing remitting disease course. In all MS subtypes miR-17 and miR-20a were significantly underexpressed in MS, confirmed by RT-PCR. We demonstrate that these miRNAs modulate T cell activation genes in a knock-in and knock-down T cell model. The same T cell activation genes are also up-regulated in MS whole blood mRNA, suggesting these miRNAs or their analogues may provide useful targets for new therapeutic approaches

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Clinical sciences
Research Field:Medical genetics (excl. cancer genetics)
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Foote, SJ (Professor Simon Foote)
UTAS Author:Stankovich, J (Dr Jim Stankovich)
UTAS Author:Taylor, BV (Professor Bruce Taylor)
ID Code:65292
Year Published:2010
Web of Science® Times Cited:214
Deposited By:Menzies Institute for Medical Research
Deposited On:2010-10-27
Last Modified:2011-06-15
Downloads:369 View Download Statistics

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