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Effect of Heparin on APP Metabolism and Aβ Production in Cortical Neurons


Klaver, D and Hung, AC and Gasperini, R and Foa, L and Aguilar, MI and Small, DH, Effect of Heparin on APP Metabolism and Aβ Production in Cortical Neurons, Neurodegenerative Diseases, 7, (1-3) pp. 187-189. ISSN 1660-2854 (2010) [Refereed Article]

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Copyright Statement

Copyright © 2010 S. Karger AG, Basel

DOI: doi:10.1159/000295661


Background: The ‚-site APP cleaving enzyme 1 (BACE1) is a major target for drug design in Alzheimerís disease. BACE1 binds strongly to heparin and other glycosaminoglycans, and there is evidence that the enzyme may interact with proteoglycans in vivo. Several studies suggest that heparin or heparan sulfate analogues may have value as therapeutic agents for the treatment of AD. Objective: To determine whether heparin can inhibit A‚ production in cortical neurons by inhibiting BACE1. Methods: Cortical neurons from APP (SW) Tg2576 mice were incubated with heparin and the amount of APP processing and A‚ production were measured by enzyme-linked immunosorbent assay and Western blotting. Results: Treatment of cortical neurons with heparin inhibited A‚ secretion. However, this effect was not mediated via inhibition of BACE1. Conclusions: Heparin or other glycosaminoglycans may have value for the treatment of Alzheimerís disease. However, the data do not support the view that a heparin-induced decrease in A‚ secretion is due to inhibition of BACE1.

Item Details

Item Type:Refereed Article
Keywords:Secretase; Amyloid; Glycosaminoglycan; Heparin; Autocatalysis
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Neurosciences not elsewhere classified
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Klaver, D (Mr David Klaver)
UTAS Author:Hung, AC (Dr Amos Hung)
UTAS Author:Gasperini, R (Dr Rob Gasperini)
UTAS Author:Foa, L (Professor Lisa Foa)
UTAS Author:Small, DH (Professor David Small)
ID Code:65236
Year Published:2010
Web of Science® Times Cited:9
Deposited By:Menzies Institute for Medical Research
Deposited On:2010-10-19
Last Modified:2012-03-01

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