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Comprehensive two-dimensional liquid chromatography: Ion chromatography x reversed-phase liquid chromatography for separation of low-molecular-mass organic acids

journal contribution
posted on 2023-05-17, 03:19 authored by Brudin, SS, Robert ShellieRobert Shellie, Paul HaddadPaul Haddad, Schoenmakers, PJ
In the work presented here a novel approach to comprehensive two-dimensional liquid chromatography is evaluated. Ion chromatography is chosen for the first-dimension separation and reversed-phase liquid chromatography is chosen for the second-dimension separation mode. The coupling of these modes is made possible by neutralising the first-dimension effluent, containing KOH, prior to transfer to the second-dimension reversed-phase column. A test mixture of 24 low-molar-mass organic acids is used for optimisation of the system. Three food and beverage samples were analysed in order to evaluate the developed methodology, the resulting two-dimensional separation is near-orthogonal, the set-up is simple and all instrumental components are available commercially. The method proved to be robust and suitable for the analysis of wine, orange juice and yogurt.

Funding

Australian Research Council

History

Publication title

Journal of Chromatography A

Volume

1217

Issue

43

Pagination

6742-6746

ISSN

0021-9673

Department/School

School of Natural Sciences

Publisher

Elsevier Science Bv

Place of publication

Po Box 211, Amsterdam, Netherlands, 1000 Ae

Rights statement

The definitive version is available at http://www.sciencedirect.com

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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    University Of Tasmania

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