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In the work presented here a novel approach to comprehensive two-dimensional liquid chromatography is evaluated. Ion chromatography is chosen for the first-dimension separation and reversed-phase liquid chromatography is chosen for the second-dimension separation mode. The coupling of these modes is made possible by neutralising the first-dimension effluent, containing KOH, prior to transfer to the second-dimension reversed-phase column. A test mixture of 24 low-molar-mass organic acids is used for optimisation of the system. Three food and beverage samples were analysed in order to evaluate the developed methodology, the resulting two-dimensional separation is near-orthogonal, the set-up is simple and all instrumental components are available commercially. The method proved to be robust and suitable for the analysis of wine, orange juice and yogurt.

Item Type:	Refereed Article
Keywords:	Ion chromatography; Reversed phase; Organic acids; Multidimensional liquid chromatography; Orthogonal separation
Research Division:	Chemical Sciences
Research Group:	Analytical Chemistry
Research Field:	Separation Science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding Knowledge
Objective Field:	Expanding Knowledge in the Chemical Sciences
Author:	Brudin, SS (Ms Stella Brudin)
Author:	Shellie, RA (Associate Professor Robert Shellie)
Author:	Haddad, PR (Professor Paul Haddad)
ID Code:	65180
Year Published:	2010
Funding Support:	Australian Research Council (DP0663781)
Web of Science® Times Cited:	21
Deposited By:	Austn Centre for Research in Separation Science
Deposited On:	2010-10-11
Last Modified:	2014-11-21
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