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Comprehensive two-dimensional liquid chromatography: Ion chromatography x reversed-phase liquid chromatography for separation of low-molecular-mass organic acids

Citation

Brudin, SS and Shellie, RA and Haddad, PR and Schoenmakers, PJ, Comprehensive two-dimensional liquid chromatography: Ion chromatography x reversed-phase liquid chromatography for separation of low-molecular-mass organic acids, Journal of Chromatography A, 1217, (43) pp. 6742-6746. ISSN 0021-9673 (2010) [Refereed Article]


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DOI: doi:10.1016/j.chroma.2010.05.064

Abstract

In the work presented here a novel approach to comprehensive two-dimensional liquid chromatography is evaluated. Ion chromatography is chosen for the first-dimension separation and reversed-phase liquid chromatography is chosen for the second-dimension separation mode. The coupling of these modes is made possible by neutralising the first-dimension effluent, containing KOH, prior to transfer to the second-dimension reversed-phase column. A test mixture of 24 low-molar-mass organic acids is used for optimisation of the system. Three food and beverage samples were analysed in order to evaluate the developed methodology, the resulting two-dimensional separation is near-orthogonal, the set-up is simple and all instrumental components are available commercially. The method proved to be robust and suitable for the analysis of wine, orange juice and yogurt.

Item Details

Item Type:Refereed Article
Keywords:Ion chromatography; Reversed phase; Organic acids; Multidimensional liquid chromatography; Orthogonal separation
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Brudin, SS (Ms Stella Brudin)
Author:Shellie, RA (Associate Professor Robert Shellie)
Author:Haddad, PR (Professor Paul Haddad)
ID Code:65180
Year Published:2010
Funding Support:Australian Research Council (DP0663781)
Web of Science® Times Cited:18
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2010-10-11
Last Modified:2014-11-21
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