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Comprehensive two-dimensional liquid chromatography: Ion chromatography x reversed-phase liquid chromatography for separation of low-molecular-mass organic acids
Citation
Brudin, SS and Shellie, RA and Haddad, PR and Schoenmakers, PJ, Comprehensive two-dimensional liquid chromatography: Ion chromatography x reversed-phase liquid chromatography for separation of low-molecular-mass organic acids, Journal of Chromatography A, 1217, (43) pp. 6742-6746. ISSN 0021-9673 (2010) [Refereed Article]
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DOI: doi:10.1016/j.chroma.2010.05.064
Abstract
In the work presented here a novel approach to comprehensive two-dimensional liquid chromatography is evaluated. Ion chromatography is chosen for the first-dimension separation and reversed-phase liquid chromatography is chosen for the second-dimension separation mode. The coupling of these modes is made possible by neutralising the first-dimension effluent, containing KOH, prior to transfer to the second-dimension reversed-phase column. A test mixture of 24 low-molar-mass organic acids is used for optimisation of the system. Three food and beverage samples were analysed in order to evaluate the developed methodology, the resulting two-dimensional separation is near-orthogonal, the set-up is simple and all instrumental components are available commercially. The method proved to be robust and suitable for the analysis of wine, orange juice and yogurt.
Item Details
Item Type: | Refereed Article |
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Keywords: | Ion chromatography; Reversed phase; Organic acids; Multidimensional liquid chromatography; Orthogonal separation |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Brudin, SS (Ms Stella Brudin) |
UTAS Author: | Shellie, RA (Associate Professor Robert Shellie) |
UTAS Author: | Haddad, PR (Professor Paul Haddad) |
ID Code: | 65180 |
Year Published: | 2010 |
Funding Support: | Australian Research Council (DP0663781) |
Web of Science® Times Cited: | 30 |
Deposited By: | Austn Centre for Research in Separation Science |
Deposited On: | 2010-10-11 |
Last Modified: | 2014-11-21 |
Downloads: | 1 View Download Statistics |
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