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CZE and ESI-MS of borate-sugar complexes


Quirino, JP, CZE and ESI-MS of borate-sugar complexes, Chromatographia, 72, (5-6) pp. 503-510. ISSN 0009-5893 (2010) [Refereed Article]

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DOI: doi:10.1365/s10337-010-1685-8


Capillary zone electrophoresis (CZE) with electrospray ionization (ESI) mass spectrometry (MS) was used to study borate (B−1) and sugar (L) complexes (L x B−1). Boric acid was adjusted to pH 10 with ammonium hydroxide to create an ESI-MS compatible CZE background electrolyte. We show for the first time that the electrophoretic peaks for each injected sugar contained both the substrates (i.e., sugar and/or multimers) and products (i.e., L x B−1). The effects of sheath liquid, temperature, and borate concentration were studied. The molecular mass information obtained from the ESI-MS provided new evidence on the mechanisms of borate-sugar complexation. Direct infusion ESI-MS and CZE-ESI-MS experiments strongly suggest that the formation of L x B−1 was from the direct reaction of a sugar or sugar multimer (L x ) and B−1. Larger L x B−1, where x > 2 were observed. Separation in the CZE dimension allows for the simultaneous analysis of a sugar mixture and simplified the ESI-MS analysis of sugars of the same molecular mass. The increase in sugar electrophoretic mobility caused by the increase in borate concentration was discussed in terms of the formation of L x B−1 complexes. In addition, the separation of five nucleosides by CZE using a borate electrolyte and detection using ESI-MS is demonstrated.

Item Details

Item Type:Refereed Article
Keywords:Capillary zone electrophoresis - ESI-MS - Sugars
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:65166
Year Published:2010
Web of Science® Times Cited:6
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2010-10-08
Last Modified:2014-11-21

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