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Identification of deletion mutants with improved performance under water-limited environments in rice (Oryza sativa L.)
Citation
Cairns, JE and Botwright Acuna, TL and Simborio, FA and Dimayuga, G and Lakshmi Praba, M and Leung, H and Torres, R and Lafitte, HR, Identification of deletion mutants with improved performance under water-limited environments in rice (Oryza sativa L.), Field Crops Research, 114, (1) pp. 159-168. ISSN 0378-4290 (2009) [Refereed Article]
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DOI: doi:10.1016/j.fcr.2009.07.019
Abstract
Rice (Oryza sativa L.) is a semi-aquatic member of the grass family that is poorly adapted to dry environments and has greater sensitivity to water-deficits than other important cereals in this family. To increase productivity in aerobic or water-limited environments rice must overcome its adaptations to flooded environments. Deletion mutants offer an alternative genetic resource for improving drought tolerance. Almost 3500 IR64 deletion mutants were screened under vegetative and reproductive stage drought stress in the field and evaluated for leaf drying and/or grain yield. Seven novel conditional mutants of rice which showed gain of function through continued growth as drought stress developed compared to the wild type were identified. Mutant recovery rate was 0.1%. Further evaluation of putative drought mutants revealed that their average shoot biomass at maturity and grain yield per plant under stress exceeded those of the wild type by two-fold. Studies under controlled conditions confirmed mutants to have continued growth of both roots and shoots as drought developed compared to the wild type, and a tendency for greater water extraction. We propose that deletions in these mutants have affected a regulator of the highly conservative growth response common to irrigated lowland rice cultivars. Our results suggest that screening deletion mutants for performance under managed drought stress in the field could be a highly effective way to identify valuable genetic resources for improved drought response and aerobic adaptation in rice.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Rice; Oryza sativa L.; Drought stress; Deletion mutants; Aerobic adaptation |
| Research Division: | Agricultural, Veterinary and Food Sciences |
| Research Group: | Crop and pasture production |
| Research Field: | Agronomy |
| Objective Division: | Plant Production and Plant Primary Products |
| Objective Group: | Grains and seeds |
| Objective Field: | Rice |
| UTAS Author: | Botwright Acuna, TL (Associate Professor Tina Acuna) |
| ID Code: | 64987 |
| Year Published: | 2009 |
| Web of Science® Times Cited: | 6 |
| Deposited By: | Agricultural Science |
| Deposited On: | 2010-09-21 |
| Last Modified: | 2012-01-20 |
| Downloads: | 1 View Download Statistics |
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