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Stability of Myrmecia pilosula (Jack Jumper) Ant venom for use in immunotherapy

Citation

Wiese, MD and Davies, NW and Chataway, TK and Milne, RW and Brown, SGA and Heddle, RJ, Stability of Myrmecia pilosula (Jack Jumper) Ant venom for use in immunotherapy, Journal of Pharmaceutical and Biomedical Analysis, 54, (2) pp. 303-310. ISSN 0731-7085 (2011) [Refereed Article]


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DOI: doi:10.1016/j.jpba.2010.08.024

Abstract

Allergy to Myrmecia pilosula (Jack Jumper Ant) venom is common in Australia, affecting ∼2.7% of some communities. Venom immunotherapy is a highly effective treatment, but for the venom to be widely distributed for clinical use, the stability and shelf-life of formulated Jack Jumper Ant venom must be demonstrated. HPLC–UV, ELISA Inhibition, SDS-PAGE and SDS-PAGE Immunoblot were used to assess venom stability under conditions of varying temperature, pH and in the presence of various stabilising agents. Optimal stability occurred between pH 8 and 10, however the presence of benzyl alcohol within this pH range resulted in a cloudy appearance within 3 days, so a pH of 6 was used. Increasing polysorbate 80 concentrations accelerated the degradation of allergenic peptides in 100ug/mL venom, but improved stability at concentrations of 1ug/mL or less. Sucrose reduced degradation of allergens Myr p 1 and Myr p 3, whilst glycerol was destabilising. In the presence of 22% sucrose, 1.1mg/mL Jack Jumper Ant venom was stable at −18 C and 4 C for 12 months; following dilution to 100ug/mL with 0.9% sodium chloride, 10mM phosphate (pH 6), 0.05% polysorbate 80 and 0.9% benzyl alcohol (giving 2% sucrose), venom was stable for 7 days when stored at 4 ◦C. Concentrated Jack Jumper Ant venom can be stored in 22% sucrose for 12 months, and after dilution to 100ug/mL for clinical use, it should be discarded after 7 days.

Item Details

Item Type:Refereed Article
Keywords:Myrmecia pilosula, Hymenoptera venom stability, ant venom immunotherapy, HPLC-UV, SDS-PAGE immunoblot
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Allergy
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Immune System and Allergy
Author:Davies, NW (Associate Professor Noel Davies)
ID Code:64734
Year Published:2011
Web of Science® Times Cited:5
Deposited By:Central Science Laboratory
Deposited On:2010-08-19
Last Modified:2017-11-02
Downloads:0

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