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Lack of evidence for recombination or spatial structure in Phoma ligulicola var. inoxydabilis populations from Australian pyrethrum fields


Pethybridge, SJ and Scott, JB and Hay, FS, Lack of evidence for recombination or spatial structure in Phoma ligulicola var. inoxydabilis populations from Australian pyrethrum fields, Plant Disease: An International Journal of Applied Plant Pathology, 96, (5) pp. 746-751. ISSN 0191-2917 (2012) [Refereed Article]

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Copyright Statement

Copyright 2012 The American Phytopathological Society

DOI: doi:10.1094/PDIS-07-10-0551


Ray blight, caused by Phoma ligulicola var. inoxydabilis, causes substantial annual losses in Australian pyrethrum fields. Fifty-nine P. ligulicola var. inoxydabilis isolates were randomly selected from fields in three distinct geographical regions in Tasmania, Australia. Genetic diversity was characterized using random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP). Based on genetic similarities of less than 99%, 56 distinct genotypes (putative clones) were observed. Mean haploid gene diversity of clone-corrected populations ranged between 0.05 and 0.31, and 0.11 and 0.32, for the RAPD and AFLP data sets, respectively. Cluster analysis indicated two distinct groups of isolates supported by all bootstrap replicates. The first cluster contained all but four isolates with representatives from all three populations. The second cluster contained two isolates from the Western and Central populations, respectively, while the remaining isolates were not able to be grouped with any distinct cluster. Analysis of the population structure suggested no evidence for spatial autocorrelation at the smallest distance classes. The presence of linkage disequilibrium was indicated regardless of population scale. Collectively, these findings provided further evidence for the absence or minor role of the teleomorph in the epidemiology of ray blight in Australian pyrethrum fields.

Item Details

Item Type:Refereed Article
Keywords:Didymella ligulicola
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Horticultural production
Research Field:Horticultural crop protection (incl. pests, diseases and weeds)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Industrial crops
Objective Field:Plant extract crops
UTAS Author:Pethybridge, SJ (Dr Sarah Pethybridge)
UTAS Author:Scott, JB (Dr Jason Scott)
UTAS Author:Hay, FS (Dr Frank Hay)
ID Code:64640
Year Published:2012
Funding Support:Australian Research Council (LP0560562)
Web of Science® Times Cited:8
Deposited By:Agricultural Science
Deposited On:2010-08-13
Last Modified:2015-06-25
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