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A micropropagation protocol for Melaleuca alternifolia (tea tree)

Citation

List, S and Brown, PH and Low, CS and Walsh, KB, A micropropagation protocol for Melaleuca alternifolia (tea tree), Australian Journal of Experimental Agriculture, 36, (6) pp. 755-60. ISSN 0816-1089 (1996) [Refereed Article]

DOI: doi:10.1071/EA9960755

Abstract

Micropropagation of Melaleuca alternifolia (tea tree) was achieved using stem segments from mature plants. A comparison of the source of explant material, and the form of cytokinin and mineral nitrogen on the induction of multiple shoots per node is reported. Further, the effect of shoot induction treatment, the age of plant used for explants and the value of the presence of charcoal, sucrose and light on subsequent root induction in plantlets is considered. A culture procedure involving nodes sourced from 1.5 to 3 cm from the shoot tip, cultured into a shoot induction medium of Murashige and Skoog (MS) basal salts supplemented with 4.5 μmol 6-benzylaminopurine/L and 60 mmol sucrose/L for 12 weeks, and then subcultured for root induction on medium containing MS basal salts, 60 mmol sucrose/L and 0.1 or 1 μmol indole-acetic acid/L for 8 weeks, is recommended.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Horticultural Production
Research Field:Horticultural Production not elsewhere classified
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other Plant Production and Plant Primary Products
Objective Field:Plant Production and Plant Primary Products not elsewhere classified
Author:Brown, PH (Professor Phil Brown)
ID Code:6462
Year Published:1996
Web of Science® Times Cited:5
Deposited By:Agricultural Science
Deposited On:1996-08-01
Last Modified:2011-08-16
Downloads:0

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