Gene for the catalytic subunit of mouse DNA-dependent protein kinase maps to the scid locus

The gene encoding the catalytic subunit of DNA-dependent protein kinase (DNA-PK(cs)) has been proposed recently as a candidate gene for the mouse severe combined immune deficiency (scid) locus. We have used a partial cDNA clone for human DNA-PK(cs) to map the mouse homologue using a large interspecific backcross panel. We found that the mouse gene for DNA-PK(cs) does not recombine with scid, consistent with the hypothesis that scid is a mutation in the mouse gene for DNA-PK(cs).