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Targeted disruption of the catalytic subunit of the DNA-PK gene in mice confers severe combined immunodeficiency and radiosensitivity
Citation
Taccioli, GE and Amatucci, AG and Beamish, HJ and Gell, D and Xiang, XH and Torres Arzayus, MI and Priestley, A and Jackson, SP and Marshak Rothstein, A and Jeggo, PA and Herrera, VL, Targeted disruption of the catalytic subunit of the DNA-PK gene in mice confers severe combined immunodeficiency and radiosensitivity , Immunity, 9, (3) pp. 355-366. ISSN 1074-7613 (1998) [Refereed Article]
DOI: doi:10.1016/S1074-7613(00)80618-4
Abstract
The DNA-dependent protein kinase is a mammalian protein complex composed of Ku70, Ku80, and DNA-PKcs subunits that has been implicated in DNA double- strand break repair and V(D)J recombination. Here, by gene targeting, we have constructed a mouse with a disruption in the kinase domain of DNA-PKcs, generating an animal model completely devoid of DNA-PK activity. Our results demonstrate that DNA-PK activity is required for coding but not for signal join formation in mice. Although our DNA-PKcs defective mice closely resemble Scid mice, they differ by having elevated numbers of CD4+CD8+ thymocytes. This suggests that the Scid mice may not represent a null phenotype and may retain some residual DNA-PKcs function.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Biological Sciences |
| Research Group: | Biochemistry and cell biology |
| Research Field: | Enzymes |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the biological sciences |
| UTAS Author: | Gell, D (Dr David Gell) |
| ID Code: | 64589 |
| Year Published: | 1998 |
| Web of Science® Times Cited: | 265 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2010-08-12 |
| Last Modified: | 2011-08-10 |
| Downloads: | 0 |
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