eCite Digital Repository

A novel haem-binding interface in the 22 kDa haem-binding protein p22HBP


Gell, DA and Westman, BJ and Gorman, D and Liew, C and Welch, JJ and Weiss, MJ and Mackay, JP, A novel haem-binding interface in the 22 kDa haem-binding protein p22HBP, Journal of Molecular Biology, 362, (2) pp. 287-297. ISSN 0022-2836 (2006) [Refereed Article]

DOI: doi:10.1016/j.jmb.2006.07.010


The 22 kDa haem-binding protein, p22HBP, is highly expressed in erythropoietic tissues and binds to a range of metallo- and non-metalloporphyrin molecules with similar affinities, suggesting a role in haem regulation or synthesis. We have determined the three-dimensional solution structure of p22HBP and mapped the porphyrin-binding site, which comprises a number of loops and a α-helix all located on a single face of the molecule. The structure of p22HBP is related to the bacterial multi-drug resistance protein BmrR, and is the first protein with this fold to be identified in eukaryotes. Strikingly, the porphyrin-binding site in p22HBP is located in a similar position to the drug-binding site of BmrR. These similarities suggest that the broad ligand specificity observed for both BmrR and p22HBP may result from a conserved ligand interaction mechanism. Taken together, these data suggest that the both the fold and its associated function, that of binding to a broad range of small hydrophobic molecules, are ancient, and have been adapted throughout evolution for a variety of purposes. © 2006 Elsevier Ltd. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Structural biology (incl. macromolecular modelling)
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Gell, DA (Dr David Gell)
ID Code:64535
Year Published:2006
Web of Science® Times Cited:7
Deposited By:Menzies Institute for Medical Research
Deposited On:2010-08-12
Last Modified:2011-07-29

Repository Staff Only: item control page