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A novel haem-binding interface in the 22 kDa haem-binding protein p22HBP
Citation
Gell, DA and Westman, BJ and Gorman, D and Liew, C and Welch, JJ and Weiss, MJ and Mackay, JP, A novel haem-binding interface in the 22 kDa haem-binding protein p22HBP, Journal of Molecular Biology, 362, (2) pp. 287-297. ISSN 0022-2836 (2006) [Refereed Article]
DOI: doi:10.1016/j.jmb.2006.07.010
Abstract
The 22 kDa haem-binding protein, p22HBP, is highly expressed in erythropoietic tissues and binds to a range of metallo- and non-metalloporphyrin molecules with similar affinities, suggesting a role in haem regulation or synthesis. We have determined the three-dimensional solution structure of p22HBP and mapped the porphyrin-binding site, which comprises a number of loops and a α-helix all located on a single face of the molecule. The structure of p22HBP is related to the bacterial multi-drug resistance protein BmrR, and is the first protein with this fold to be identified in eukaryotes. Strikingly, the porphyrin-binding site in p22HBP is located in a similar position to the drug-binding site of BmrR. These similarities suggest that the broad ligand specificity observed for both BmrR and p22HBP may result from a conserved ligand interaction mechanism. Taken together, these data suggest that the both the fold and its associated function, that of binding to a broad range of small hydrophobic molecules, are ancient, and have been adapted throughout evolution for a variety of purposes. © 2006 Elsevier Ltd. All rights reserved.
Item Details
Item Type: | Refereed Article |
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Research Division: | Biological Sciences |
Research Group: | Biochemistry and cell biology |
Research Field: | Structural biology (incl. macromolecular modelling) |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Gell, DA (Dr David Gell) |
ID Code: | 64535 |
Year Published: | 2006 |
Web of Science® Times Cited: | 7 |
Deposited By: | Menzies Institute for Medical Research |
Deposited On: | 2010-08-12 |
Last Modified: | 2011-07-29 |
Downloads: | 0 |
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