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Fast analysis of phenolic acids by electrokinetic supercharging-nonaqueous capillary electrophoresis


Lu, Y and Breadmore, MC, Fast analysis of phenolic acids by electrokinetic supercharging-nonaqueous capillary electrophoresis, Journal of Separation Science, 33, (14) pp. 2140-2144. ISSN 1615-9306 (2010) [Refereed Article]

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DOI: doi:10.1002/jssc.201000090


A method was developed to analyze phenolic acids by nonaqueous CE after online concentration with electrokinetic supercharging. The EOF was reversed using a polyelectrolyte multilayer approach based on the successive adsorption of poly(diallyldimethylamonium chloride) and poly(styrenesulfonate) to reduce the analysis time. The results showed that the coatings were stable during 40 consecutive injections. Four phenolic acids were separated within 8 min using 30 mM ammonium acetate (pH* 8.0). The electrokinetic injection time and terminator length of the electrokinetic supercharging method were optimized to improve the detection sensitivity. Under the optimized conditions (electrokinetic injection of 100 s, -10 kV; terminator of 20 mM 2-(cyclohexylamino) ethanesulfonic acid, 22 s, 0.5 psi), the sensitivity was enhanced from 300- to 440-fold. The detection limits, based on three times noise, were in the range 1.0-2.5 ng/mL.

Item Details

Item Type:Refereed Article
Keywords:Electrokinetic supercharging • Nonaqueous CE • Phenolic acids
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Lu, Y (Dr Yuanqi Lu)
UTAS Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:64305
Year Published:2010
Funding Support:Australian Research Council (DP0984745)
Web of Science® Times Cited:21
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2010-07-15
Last Modified:2015-02-07

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