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Determination of food grade antioxidants using microemulsion electrokinetic chromatography
journal contribution
posted on 2023-05-17, 02:52 authored by Darji, V, Boyce, MC, Bennett, I, Michael BreadmoreMichael Breadmore, Joselito QuirinoSeven food grade antioxidants were resolved by microemulsion electrokinetic chromatography. The antioxidants included propyl gallate, octyl gallate, dodecyl gallate, butylated hydroxyanisole, butylated hydroxytoluene, tert-butylhydroquinone and -tocopherol. They were resolved in 11 min using a low pH microemulsion containing 20% propan-2-ol. Theoretical plates in the range 39 000-95 000 per length of column (38.5 cm) were recorded for the antioxidants. The LODs were calculated to be in the range 9-44 g/mL (30 s hydrostatic injection and a 50 m id capillary column). The method can also be used to simultaneously analyse for the synthetic preservatives sorbic acid, benzoic acid, salicylic acid and the parabens. The method was successfully applied to noodles for the determination of butylated hydroxytoluene and propyl gallate and to pharmaceutical supplement tablets for -tocopherol. The in-capillary concentration of butylated hydroxytoluene by sweeping was also demonstrated.
Funding
Australian Research Council
History
Publication title
ElectrophoresisVolume
31Issue
13Pagination
2267-2271ISSN
0173-0835Department/School
School of Natural SciencesPublisher
Wiley-V C H Verlag GmbhPlace of publication
Po Box 10 11 61, Weinheim, Germany, D-69451Rights statement
The definitive published version is available online at: http://interscience.wiley.comRepository Status
- Restricted