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Wild barley-specific genomic libraries were developed for the purpose of creating a ‘comprehensive’ genomic representation of the primary Hordeum genepool capable of more robust genotyping of barley. In order to enrich for wild barley-specific sequences in the DArT libraries, suppression subtraction hybridization (SSH) was performed using cultivated barley as the subtraction driver and wild barley as the tester. Four doubled-haploid populations were genotyped with the comprehensive barley DArT array, including two from wild × cultivated crosses (Damon/Harrington and Shechem/Harrington) and two from cultivated × cultivated crosses (Albacete/Barbarrouse and TX9425/Naso Nijo). Analysis of genotyping data revealed that the SSH process was somewhat ineffective at enriching for unique sequences in this application of DArT marker development. However, the addition of markers derived from wild barley proved to be an effective means for increasing the number of polymorphic markers obtainable from a single DArT assay. Genetic maps of the four component populations were developed and 607 newly developed DArT markers were integrated with a barley consensus map to create a new synthetic map of the barley genome containing 3542 markers. This significantly increased the resolution of the consensus map and improved the power of the map to provide a reference for profiling genetic diversity within the primary Hordeum genepool. The improvement in the genotyping capability of the comprehensive DArT genomic representation and the higher resolution of the synthetic map facilitates an even greater flexibility of DArT markers to be utilized as a fast, high-throughput platform for molecular marker-based barley breeding.

Item Type:	Refereed Article
Keywords:	Hordeum vulgare subsp. spontaneum – Synthetic map – Diversity arrays technology
Research Division:	Agricultural and Veterinary Sciences
Research Group:	Crop and Pasture Production
Research Field:	Crop and Pasture Improvement (Selection and Breeding)
Objective Division:	Plant Production and Plant Primary Products
Objective Group:	Winter Grains and Oilseeds
Objective Field:	Barley
Author:	Zhou, M (Professor Meixue Zhou)
ID Code:	64235
Year Published:	2011
Web of Science® Times Cited:	24
Deposited By:	Agricultural Science
Deposited On:	2010-07-08
Last Modified:	2011-04-14
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