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Engineering greater aluminium resistance in wheat by over-expressing TaALMT1


Pereira, JF and Zhou, G and Delhaize, E and Richardson, T and Zhou, M and Ryan, PR, Engineering greater aluminium resistance in wheat by over-expressing TaALMT1, Annals of Botany, 106, (1) pp. 205-214. ISSN 0305-7364 (2010) [Refereed Article]

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DOI: doi:10.1093/aob/mcq058


Background and Aims: Expected increases in world population will continue to make demands on agricultural productivity and food supply. These challenges will only be met by increasing the land under cultivation and by improving the yields obtained on existing farms. Genetic engineering can target key traits to improve crop yields and to increase production on marginal soils. Soil acidity is a major abiotic stress that limits plant production worldwide. The goal of this study was to enhance the acid soil tolerance of wheat by increasing its resistance to Al3+ toxicity. Methods: Particle bombardment was used to transform wheat with TaALMT1, the Al3+ resistance gene from wheat, using the maize ubiquitin promoter to drive expression. TaALMT1 expression, malate efflux and Al3+ resistance were measured in the T1 and T2 lines and compared with the parental line and an Al3+-resistant reference genotype, ET8. Key Results: Nine T2 lines showed increased TaALMT1 expression, malate efflux and Al3+ resistance when compared with untransformed controls and null segregant lines. Some T2 lines displayed greater Al3+ resistance than ET8 in both hydroponic and soil experiments. Conclusions: The Al3+ resistance of wheat was increased by enhancing TaALMT1 expression with biotechnology. This is the first report of a major food crop being stably transformed for greater Al3+ resistance. Transgenic strategies provide options for increasing food supply on acid soils.

Item Details

Item Type:Refereed Article
Keywords:Acid soil, aluminium resistance, TaALMT1 gene, transgenic wheat, Triticum aestivum, aluminum
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Crop and pasture improvement (incl. selection and breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Grains and seeds
Objective Field:Wheat
UTAS Author:Zhou, G (Mr Gaofeng Zhou)
UTAS Author:Zhou, M (Professor Meixue Zhou)
ID Code:64233
Year Published:2010
Web of Science® Times Cited:77
Deposited By:Agricultural Science
Deposited On:2010-07-07
Last Modified:2011-04-14

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