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Focal Damage to the Adult Rat Neocortex Induces Wound Healing Accompanied by Axonal Sprouting and Dendritic Structural Plasticity

Citation

Blizzard, CA and Chuckowree, JA and King, AE and Hosie, KA and McCormack, GH and Chapman, JA and Vickers, JC and Dickson, TC, Focal Damage to the Adult Rat Neocortex Induces Wound Healing Accompanied by Axonal Sprouting and Dendritic Structural Plasticity , Cerebral Cortex, 21, (2) pp. 281-291. ISSN 1047-3211 (2011) [Refereed Article]


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The definitive publisher-authenticated version is available online at: www.oxfordjournals.org

DOI: doi:10.1093/cercor/bhq091

Abstract

Accumulating evidence indicates that damage to the adult mammalian brain evokes an array of adaptive cellular responses and may retain a capacity for structural plasticity. We have investigated the cellular and architectural alterations following focal experimental brain injury, as well as the specific capacity for structural remodeling of neuronal processes in a subset of cortical interneurons. Focal acute injury was induced by transient insertion of a needle into the neocortex of anesthetized adult male Hooded-- Wistar rats and thy1 green fluorescent protein (GFP) mice. Immunohistochemical, electron microscopy, and bromodeoxyuridine cell proliferation studies demonstrated an active and evolving response of the brain to injury, indicating astrocytic but not neuronal proliferation. Immunolabeling for the neuron-specific markers phosphorylated neurofilaments, a-internexin and calretinin at 7 days post injury (DPI) indicated phosphorylated neurofilaments and a-internexin but not calretinin immunopositive axonal sprouts within the injury site. However, quantitative studies indicated a significant realignment of horizontally projecting dendrites of calretinin-labeled interneurons at 14 DPI. This remodeling was specific to calretinin immunopositive interneurons and did not occur in a subpopulation of pyramidal neurons expressing GFP in the injured mouse cortex. These data show that subclasses of cortical interneurons are capable of adaptive structural remodeling.

Item Details

Item Type:Refereed Article
Keywords:cortical injury, interneuron, neurogenesis, plasticity, regeneration
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Central Nervous System
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Nervous System and Disorders
Author:Blizzard, CA (Dr Catherine Blizzard)
Author:Chuckowree, JA (Dr Jyoti Chuckowree)
Author:King, AE (Associate Professor Anna King)
Author:Hosie, KA (Dr Katherine Southam)
Author:McCormack, GH (Mr Graeme McCormack)
Author:Chapman, JA (Dr Jamie Chapman)
Author:Vickers, JC (Professor James Vickers)
Author:Dickson, TC (Professor Tracey Dickson)
ID Code:64009
Year Published:2011
Web of Science® Times Cited:26
Deposited By:Menzies Institute for Medical Research
Deposited On:2010-06-16
Last Modified:2014-12-17
Downloads:0

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