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Focal Damage to the Adult Rat Neocortex Induces Wound Healing Accompanied by Axonal Sprouting and Dendritic Structural Plasticity
journal contribution
posted on 2023-05-17, 02:45 authored by Catherine BlizzardCatherine Blizzard, Jyoti ChuckowreeJyoti Chuckowree, Anna KingAnna King, Katherine SouthamKatherine Southam, Graeme McCormackGraeme McCormack, Jamie ChapmanJamie Chapman, James VickersJames Vickers, Tracey DicksonTracey DicksonAccumulating evidence indicates that damage to the adult mammalian brain evokes an array of adaptive cellular responses and may retain a capacity for structural plasticity. We have investigated the cellular and architectural alterations following focal experimental brain injury, as well as the specific capacity for structural remodeling of neuronal processes in a subset of cortical interneurons. Focal acute injury was induced by transient insertion of a needle into the neocortex of anesthetized adult male Hooded-- Wistar rats and thy1 green fluorescent protein (GFP) mice. Immunohistochemical, electron microscopy, and bromodeoxyuridine cell proliferation studies demonstrated an active and evolving response of the brain to injury, indicating astrocytic but not neuronal proliferation. Immunolabeling for the neuron-specific markers phosphorylated neurofilaments, a-internexin and calretinin at 7 days post injury (DPI) indicated phosphorylated neurofilaments and a-internexin but not calretinin immunopositive axonal sprouts within the injury site. However, quantitative studies indicated a significant realignment of horizontally projecting dendrites of calretinin-labeled interneurons at 14 DPI. This remodeling was specific to calretinin immunopositive interneurons and did not occur in a subpopulation of pyramidal neurons expressing GFP in the injured mouse cortex. These data show that subclasses of cortical interneurons are capable of adaptive structural remodeling.
History
Publication title
Cerebral CortexVolume
21Pagination
281-291ISSN
1047-3211Department/School
Menzies Institute for Medical ResearchPublisher
Oxford Univ Press IncPlace of publication
Journals Dept, 2001 Evans Rd, Cary, USA, Nc, 27513Rights statement
The definitive publisher-authenticated version is available online at: www.oxfordjournals.orgRepository Status
- Restricted